Fig. S7

Characterization of pathological features at the trunk level in the different grades of severity of acute organophosphorus poisoning induced by chlorpyrifos-oxon in zebrafish larvae. Lateral view of the trunk of 8 dpf zebrafish larvae control (A), grade 1 (B), grade 2 (C) and grade 3 (D) after the double whole-mount immunofluorescence with F59 (red staining) and anti-acetylated alpha tubulin (green staining) antibodies. This labelling provides information on integrity and architecture of slow muscle fibers and spinal axonal tracts, respectively. The area within the white dashed lines shows the arrangement of the slow muscle fibers in a similar region of the ventral trunk in the different phenotypes. Asterisks indicate rupture or detachments of slow muscle fibers along the dorsal trunk of a representative grade 3 larva. Lateral view of the trunk (dorsal is to the top and anterior is to the left). Scale bar: 100 µm.