Fig. S3

Morphological features on 8 days post-fertilization zebrafish larvae exhibiting moderate and severe acute organophosphorus poisoning by 24 h exposure to chlorpyrifos-oxon. (A) Lateral views of three representative larvae exhibiting grade 2 (moderate) phenotype, with stiffness, impaired relaxation of the axial muscle fibers and overinflation of the swim bladder. Arrowheads indicate folding of the apical ectodermal ridge. White asterisks indicate the over-inflated swim bladder. Arrow indicates altered jaw in a larvae resulting from the hyperactivity of the jaw muscles. (B-E) Compacted head and periocular and/or peritoneal edema are common features in grade 3 (severe) phenotype. Lateral view (B,C) and dorsal view (D,E) of control (B,D) and grade 3 (C,E) larvae. Dashed trace shows the dramatic change in the morphology of the head in grade 3 larva (C). Presence of periocular (C,E) and peritoneal (C) edema are indicated by arrows. Scale bar: 400 µm.