PUBLICATION
Zebrafish Models for Human Acute Organophosphorus Poisoning
- Authors
- Faria, M., Garcia-Reyero, N., Padrós, F., Babin, P.J., Sebastián, D., Cachot, J., Prats, E., Arick Ii, M., Rial, E., Knoll-Gellida, A., Mathieu, G., Le Bihanic, F., Escalon, B.L., Zorzano, A., Soares, A.M., Raldúa, D.
- ID
- ZDB-PUB-151023-5
- Date
- 2015
- Source
- Scientific Reports 5: 15591 (Journal)
- Registered Authors
- Babin, Patrick J., Raldúa, Demetrio, Soares, Amadeu
- Keywords
- none
- MeSH Terms
-
- Acetylcholinesterase/metabolism
- Animals
- Chemical Terrorism*
- Chlorpyrifos/toxicity*
- Disease Models, Animal
- Humans
- Organophosphate Poisoning/drug therapy*
- Organophosphate Poisoning/physiopathology
- Small Molecule Libraries/administration & dosage*
- Small Molecule Libraries/chemistry
- Zebrafish
- PubMed
- 26489395 Full text @ Sci. Rep.
Citation
Faria, M., Garcia-Reyero, N., Padrós, F., Babin, P.J., Sebastián, D., Cachot, J., Prats, E., Arick Ii, M., Rial, E., Knoll-Gellida, A., Mathieu, G., Le Bihanic, F., Escalon, B.L., Zorzano, A., Soares, A.M., Raldúa, D. (2015) Zebrafish Models for Human Acute Organophosphorus Poisoning. Scientific Reports. 5:15591.
Abstract
Terrorist use of organophosphorus-based nerve agents and toxic industrial chemicals against civilian populations constitutes a real threat, as demonstrated by the terrorist attacks in Japan in the 1990 s or, even more recently, in the Syrian civil war. Thus, development of more effective countermeasures against acute organophosphorus poisoning is urgently needed. Here, we have generated and validated zebrafish models for mild, moderate and severe acute organophosphorus poisoning by exposing zebrafish larvae to different concentrations of the prototypic organophosphorus compound chlorpyrifos-oxon. Our results show that zebrafish models mimic most of the pathophysiological mechanisms behind this toxidrome in humans, including acetylcholinesterase inhibition, N-methyl-D-aspartate receptor activation, and calcium dysregulation as well as inflammatory and immune responses. The suitability of the zebrafish larvae to in vivo high-throughput screenings of small molecule libraries makes these models a valuable tool for identifying new drugs for multifunctional drug therapy against acute organophosphorus poisoning.
Errata / Notes
In this Article, Mark Arick II and B. Lynn Escalon are incorrectly listed as being affiliated with ‘Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, MS, USA’ and ‘EPOC, UMR CNRS 5805, Université de Bordeaux, 33405 Talence, France’ respectively. The correct affiliations are listed below: Mark Arick II
Institute for Genomics, Biocomputing & Biotechnology (IGBB), Mississippi State University, Starkville, Mississippi, USA
B. Lynn Escalon
Environmental Laboratory, US Army Engineer Research and Development Center, Vicksburg, MS, USA
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping