FIGURE

Fig. 4

ID

ZDB-FIG-151120-14

Publication

Won et al., 2015 - Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas

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Fig. 4

The lnx2a^?329 mutant recapitulates the morphant phenotype. (A) Sequence of the lnx2a^?329 mutation (Fig. 3A). The lowercase sequence in the mutant indicates that it is in intron 2, with lnx2a^?329 having lost the exon 2 splice donor site. (B and C) Genotyping of lnx2a^?329 by genomic PCR (Δ329), RT-PCR (?510 equaling exon 2) (B), and by Western blotting of embryo extracts showing the presence of N-truncated protein (C). (D) Phenotypic analysis of lnx2a^?329 mutants at 48 hpf. (Scale bar: 50 �m.) (E) The quantification of pancreatic defects by analysis of ptf1a expression, classified as in Fig. 2D. Development of the exocrine pancreas is specifically suppressed in lnx2a^?329.

Expression Data

Genes:	gcga ins mnx2a ptf1a
Fish:	AB lnx2a^y447/y447
Anatomical Terms:	endocrine pancreas exocrine pancreas
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lnx2a^y447/y447 lnx2a^y447/y447 lnx2a^y447/y447
Observed In:	exocrine pancreas exocrine pancreas development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA