ZFIN ID: ZDB-FIG-151117-3
Wang et al., 2015 - Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis. Developmental Cell   34(6):621-31 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

Homing Process Is Disturbed due to the Reduced Expression of ccr9a

(A) Confocal imaging of coro1a:EGFP/lyz:DsRed embryos showing no coro1a:EGFP cells in the thymus (white circle) in irf4a morphants. Scale bar, 40 µm.

(B) Number of coro1a:EGFP cells entering the thymus every 30 min in the control and irf4a morphants (n = 6) during the homing process. p < 0.05.

(C) Quantification of embryos with coro1a:EGFP cells (>5) in the thymus of the control (n = 88) and irf4a morphants (n = 92) at 3.5 dpf.

(D) Expression of foxn1 in the control and irf4a morphants at 3 dpf.

(E) Expression of ccl25a and ccl25b in the control and irf4a morphants at 3 dpf.

(F) Expression of ccr9a in the control and irf4a morphants at 3 dpf. Red arrows indicate the thymus or perithymic region.

(G) Expression of cmyb, ikaros, and ccr9a in the CHT of the control and irf4a morphants at 2 dpf. Red arrows indicate hematopoietic progenitors expressing cmyb, ikaros, or ccr9a in the CHT region.

(H) qPCR analysis of cmyb, ikaros, and ccr9a in the control and irf4a morphants at 2 dpf. ns, not significant. p < 0.01. All data are mean ± SD.

See also Figure S3 and Movie S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ccl25a WT control Protruding-mouth thymus ISH
WT + MO1-irf4a control Protruding-mouth thymus ISH
ccl25b WT control Protruding-mouth thymus ISH
WT + MO1-irf4a control Protruding-mouth thymus ISH
ccr9a WT control Long-pec intermediate cell mass of mesoderm ISH
Protruding-mouth thymus ISH
WT + MO1-irf4a control Long-pec intermediate cell mass of mesoderm ISH
Protruding-mouth Not Detected thymus ISH
DsRed2 hkz04tTg; nz50Tg control Pec-fin neutrophil IFL
hkz04tTg; nz50Tg + MO1-irf4a control Pec-fin neutrophil IFL
Pec-fin thymus primordium neutrophil IFL
EGFP hkz04tTg; nz50Tg control Pec-fin thymus primordium IFL
hkz04tTg; nz50Tg + MO1-irf4a control Pec-fin Not Detected thymus primordium IFL
foxn1 WT control Protruding-mouth thymus ISH
WT + MO1-irf4a control Protruding-mouth thymus ISH
ikzf1 WT control Long-pec intermediate cell mass of mesoderm hematopoietic multipotent progenitor cell ISH
WT + MO1-irf4a control Long-pec intermediate cell mass of mesoderm hematopoietic multipotent progenitor cell ISH
myb WT control Long-pec intermediate cell mass of mesoderm hematopoietic multipotent progenitor cell ISH
WT + MO1-irf4a control Long-pec intermediate cell mass of mesoderm hematopoietic multipotent progenitor cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-irf4a control Long-pec intermediate cell mass of mesoderm ccr9a expression decreased amount, abnormal
Long-pec intermediate cell mass of mesoderm ccr9a expression decreased distribution, abnormal
Protruding-mouth thymus ccr9a expression absent, abnormal
hkz04tTg; nz50Tg + MO1-irf4a control Pec-fin lymphoid lineage cell migration into thymus decreased process quality, abnormal
Pec-fin thymus hematopoietic stem cell migration decreased occurrence, abnormal
Pec-fin thymus primordium EGFP expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell