ZFIN ID: ZDB-FIG-151117-2
Wang et al., 2015 - Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis. Developmental Cell   34(6):621-31 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 2

T Cell Development Is Impaired in irf4a-Deficient Embryos

(A) Location and sequence of the TALEN target site for the irf4a gene.

(B) Sequence flanking the TALEN target site in F2 embryos. The deletion of seven nucleotides is shown in the black box.

(C) Expression of rag1, lck, and tcra in the thymus (red arrow) of the siblings and irf4a mutants at 4 or 5 dpf. The embryos for WISH were obtained from an incross of genotyped heterozygous irf4a mutants.

(D) Cellular structure of the thymus in the control and irf4a morphants at 4 dpf. The transverse sections were observed with TEM. L, lymphocyte; E, thymic epithelial cells. Scale bar, 2 µm.

(E) Confocal images of cmyb:EGFP cells in the thymus of the control or irf4a morphants at 4 dpf. The white circle indicates the thymus region below the otic vesicle (OV), denoted with the white dashed line. Scale bar, 20 µm.

(F) Enumeration of cmyb:EGFP cells in the thymus of the control and irf4a morphants at 4 dpf. p < 0.001.

(G) Expression of cmyb, ikaros, and coro1a in the thymus (red arrow) of the control and irf4a morphants at 3 dpf.

(H) TUNEL assay of the control and irf4a morphants at 3 dpf. The irf4a MOatg was injected into coro1a:EGFP transgenic embryos. The white circle indicates the thymus region behind the eye (E), denoted with the white dashed line. White arrows denote apoptotic cells. Scale bar, 40 µm. All data are mean ± SD.

See also Figure S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
coro1a WT control Protruding-mouth thymus ISH
WT + MO1-irf4a control Protruding-mouth Not Detected thymus ISH
EGFP hkz04tTg control Protruding-mouth thymus IHC
hkz04tTg + MO1-irf4a control Protruding-mouth Not Detected thymus IHC
zf169Tg control Day 4 thymus hematopoietic multipotent progenitor cell IFL
zf169Tg + MO1-irf4a control Day 4 thymus hematopoietic multipotent progenitor cell IFL
ikzf1 WT control Protruding-mouth thymus ISH
WT + MO1-irf4a control Protruding-mouth Not Detected thymus ISH
lck WT control Day 4 thymus T cell ISH
irf4aioz3/ioz3 control Day 4 Not Detected thymus ISH
myb WT control Protruding-mouth thymus ISH
WT + MO1-irf4a control Protruding-mouth Not Detected thymus ISH
rag1 WT control Day 4 thymus T cell ISH
irf4aioz3/ioz3 control Day 4 Not Detected thymus ISH
trac WT control Day 5 thymus T cell ISH
irf4aioz3/ioz3 control Day 4 Not Detected thymus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-irf4a control Protruding-mouth thymus coro1a expression absent, abnormal
Protruding-mouth thymus ikzf1 expression absent, abnormal
Protruding-mouth thymus myb expression absent, abnormal
WT + MO1-irf4a standard conditions Day 4 thymus T cell absent, abnormal
hkz04tTg + MO1-irf4a control Protruding-mouth thymus EGFP expression absent, abnormal
irf4aioz3/ioz3 control Day 4 thymus lck expression absent, abnormal
Day 4 thymus rag1 expression absent, abnormal
Day 4 thymus trac expression absent, abnormal
zf169Tg + MO1-irf4a control Day 4 thymus hematopoietic multipotent progenitor cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell