ZFIN ID: ZDB-FIG-151117-5
Wang et al., 2015 - Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis. Developmental Cell   34(6):621-31 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 5

Irf4a-Deficient Lymphoid Progenitors Turn to Myeloid Cells

(A) Expression of coro1a in the siblings and irf4a mutants at 3 dpf. The black arrowhead indicates the thymus, and the red arrow indicates the coro1a-expressing cell in the CHT.

(B) Confocal images of coro1a:EGFP cells in the thymus (white circle) and CHT of the control and irf4a morphants at 3 dpf. Scale bar, 80 µm.

(C) Representative FACS analysis of whole-body coro1a:EGFP cells in the control and irf4a morphants at 3 dpf.

(D) Enumeration of whole-body coro1a:EGFP cells shown in (C). Data are representative of three independent experiments (n = 180). ns, not significant.

(E) Expression of mfap4 and lyz in the CHT of the control and irf4a morphants at 3 dpf. Red arrows indicate myeloid cells expressing mfap4 or lyz.

(F) Brief diagram of irf4a knockin construct and generation of transgenic line for single-cell tracing.

(G) Time-lapse imaging of the conversion of the irf4a-deficient cell with EGFP knockin to express lyz:DsRed in the CHT from 58 to 66 hpf. Scale bar, 10 µm.

(H) Confocal images of cmyb:EGFP/lyz:DsRed embryos showing cmyb:EGFP, lyz:DsRed, or yellow cells in the CHT of the control or irf4a morphants at 3 dpf. Scale bar, 40 µm.

(I) Enumeration of cmyb:EGFP, lyz:DsRed, or yellow cells shown in (H). p < 0.01; p < 0.05. All data are mean ± SD.

See also Figure S5 and Movie S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
coro1a WT control Protruding-mouth hematopoietic system leukocyte ISH
Protruding-mouth leukocyte ISH
Protruding-mouth thymus leukocyte ISH
irf4aioz3/ioz3 control Protruding-mouth hematopoietic system leukocyte ISH
Protruding-mouth thymus leukocyte ISH
DsRed2 nz50Tg ; irf4aioz2Tg control Long-pec to Pec-fin hematopoietic system hematopoietic cell IFL
Protruding-mouth hematopoietic system IFL
nz50Tg; zf169Tg control Protruding-mouth hematopoietic system IFL
nz50Tg; zf169Tg + MO1-irf4a control Protruding-mouth hematopoietic system IFL
EGFP hkz04tTg control Protruding-mouth hematopoietic system leukocyte IHC
Protruding-mouth thymus leukocyte IHC
hkz04tTg + MO1-irf4a control Protruding-mouth hematopoietic system leukocyte IHC
Protruding-mouth thymus leukocyte IHC
nz50Tg; zf169Tg control Protruding-mouth hematopoietic system IFL
nz50Tg; zf169Tg + MO1-irf4a control Protruding-mouth hematopoietic system IFL
GFP nz50Tg ; irf4aioz2Tg control Long-pec to Pec-fin hematopoietic system hematopoietic cell IFL
Protruding-mouth hematopoietic system IFL
lyz WT control Protruding-mouth neutrophil ISH
WT + MO1-irf4a control Protruding-mouth neutrophil ISH
mfap4 WT control Protruding-mouth macrophage ISH
WT + MO1-irf4a control Protruding-mouth macrophage ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-irf4a control Protruding-mouth intermediate cell mass of mesoderm macrophage increased amount, abnormal
Protruding-mouth intermediate cell mass of mesoderm neutrophil increased amount, abnormal
Protruding-mouth macrophage mfap4 expression increased distribution, abnormal
Protruding-mouth neutrophil lyz expression increased distribution, abnormal
hkz04tTg + MO1-irf4a control Protruding-mouth hematopoietic system leukocyte EGFP expression increased amount, abnormal
Protruding-mouth intermediate cell mass of mesoderm leukocyte increased amount, abnormal
Protruding-mouth thymus leukocyte decreased amount, abnormal
Protruding-mouth thymus leukocyte EGFP expression decreased amount, abnormal
Protruding-mouth thymus leukocyte EGFP expression decreased distribution, abnormal
irf4aioz3/ioz3 control Protruding-mouth hematopoietic system leukocyte coro1a expression increased amount, abnormal
Protruding-mouth intermediate cell mass of mesoderm leukocyte increased amount, abnormal
Protruding-mouth thymus leukocyte coro1a expression decreased amount, abnormal
Protruding-mouth thymus leukocyte decreased amount, abnormal
nz50Tg; zf169Tg + MO1-irf4a control Protruding-mouth myeloid cell differentiation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell