|ZFIN ID: ZDB-FIG-151117-5|
|Stage Range:||Long-pec to Protruding-mouth|
Irf4a-Deficient Lymphoid Progenitors Turn to Myeloid Cells
(A) Expression of coro1a in the siblings and irf4a mutants at 3 dpf. The black arrowhead indicates the thymus, and the red arrow indicates the coro1a-expressing cell in the CHT.
(B) Confocal images of coro1a:EGFP cells in the thymus (white circle) and CHT of the control and irf4a morphants at 3 dpf. Scale bar, 80 µm.
(C) Representative FACS analysis of whole-body coro1a:EGFP cells in the control and irf4a morphants at 3 dpf.
(D) Enumeration of whole-body coro1a:EGFP cells shown in (C). Data are representative of three independent experiments (n = 180). ns, not significant.
(E) Expression of mfap4 and lyz in the CHT of the control and irf4a morphants at 3 dpf. Red arrows indicate myeloid cells expressing mfap4 or lyz.
(F) Brief diagram of irf4a knockin construct and generation of transgenic line for single-cell tracing.
(G) Time-lapse imaging of the conversion of the irf4a-deficient cell with EGFP knockin to express lyz:DsRed in the CHT from 58 to 66 hpf. Scale bar, 10 µm.
(H) Confocal images of cmyb:EGFP/lyz:DsRed embryos showing cmyb:EGFP, lyz:DsRed, or yellow cells in the CHT of the control or irf4a morphants at 3 dpf. Scale bar, 40 µm.
(I) Enumeration of cmyb:EGFP, lyz:DsRed, or yellow cells shown in (H). p < 0.01; p < 0.05. All data are mean ± SD.
See also Figure S5 and Movie S2.
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell