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ZFIN ID: ZDB-FIG-151117-11
Wu et al., 2015 - Control of Wnt5b secretion by wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression. Journal of Cell Science   128(12):2328-39 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4 Wntless is required for Wnt5b secretion. (A) Cartoon showing cross-section view of the developing head of 48hpf embryos. E, eye; H, hypophysis; M, mesencephalon; P, denotes pharyngeal arch. Dorsal (D) is to the left, ventral (V) to the right. The green box indicates the pharyngeal region shown in images B–E. The red box indicates the region shown in images F–K. (B–E) Confocal images showing sections from the developing jaw of control-MO-injected (5mis-wlsMO) (B,D) wls-MO-injected (wlsMO) (C,E) embryos labeled by anti-β-catenin (in green) plus anti-Wnt5b (red in B,C) or anti-Wnt11 (red in D,E). (F–Q) Images showing enlarged pharyngeal regions from B–E. The arrow in M indicates the regions where Wnt5b is not accumulated on the cell membranes. All images were collected from embryos at 48hpf. Numbers in the bottom left corners in F–Q indicate the number of the embryos showing the indicated phenotype and the total number examined (denominator).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
wnt5b Ab1-wnt5b AB control Long-pec Not Detected pharyngeal arch cytoplasm IHC
Ab1-wnt5b Long-pec pharyngeal arch plasma membrane IHC
Ab1-wnt5b AB + MO1-wls standard conditions Long-pec pharyngeal arch cytoplasm IHC
Ab1-wnt5b Long-pec Not Detected pharyngeal arch plasma membrane IHC
wnt11 Ab1-wnt11 AB control Long-pec Not Detected pharyngeal arch cytoplasm IHC
Ab1-wnt11 Long-pec pharyngeal arch plasma membrane IHC
Ab1-wnt11 AB + MO1-wls standard conditions Long-pec Not Detected pharyngeal arch cytoplasm IHC
Ab1-wnt11 Long-pec pharyngeal arch plasma membrane IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-ctnnb IHC AB control Long-pec Not Detected pharyngeal arch cytoplasm
IHC Long-pec pharyngeal arch plasma membrane
IHC AB + MO1-wls standard conditions Long-pec Not Detected pharyngeal arch cytoplasm
IHC Long-pec pharyngeal arch plasma membrane
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-wls standard conditions Long-pec pharyngeal arch Wnt protein secretion decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.