Fig. 3

Ectopic expression of full-length fgf3 mRNAs rescues the jaw cartilage defects in wls morphants. (A,B) fgf3 expression in (A) control-MO-injected (5mis-wls^MO), and (B) wls-MO-injected (wls^MO) embryos. Brackets indicate the pharyngeal pouches where fgf3 expression is reduced. i, isthmus; os, optic stalk; pvh, posterior-ventral hypothalamus. (C,D) Enlarged pharyngeal regions from images in A,B. White dotted lines mark the regions of anterior or posterior pouches. (E) Signal intensity analyses for images shown in C and D. (F,G) fgf8 expressions in (F) 5mis-wls^MO or (G) wls^MO embryos at 48h. (H–J) Images of enlarged head regions from fgf3 mRNA and wls^MO double-injected (wls^MO+fgf3) larvae at 96h. Arrows indicate the Meckle′s cartilages. (K) Rescue of jaw defects by fgf3 mRNAs in wls^MO larvae. The percentages of normal (blue bar) and abnormal (green bar) morphology are 94.74% and 5.26% with 5mis-wls^MO; 1.22% and 98.78% with wls^MO; 67.24%, 32.76% with wls^MO and fgf3 mRNA co-injection; and 3.4% and 96.6% with wls^MO and fgf8 mRNA co-injection. Phenotypes of the embryos were scored at 4dpf from three independent experiments. (L) Cartilage position analyses. Definition for A, B and C lines are described in the Materials and Methods. Refer to Fig. 3Q for the definition of line A, B and C. The B:A ratios (B/A) are 1.23 in 5mis-wls^MO embryos, 0.86 in wls^MO embryos, 1.11 in wlsl^MO and fgf3 mRNA co-injected embryos and 0.85 in wls^MO and fgf8 mRNA co-injected embryos. The C:A ratios (C/A) are 0.66 in 5mis-wls^MO embryos, 0.31 in wls^MO embryos, 0.55 in wls^MO and fgf3 mRNA co-injected embryos. Lateral views are shown for images in A–D, ventral views are shown in E,G. Numbers in the bottom left corners in A, B, F and G indicate the number of the embryos showing the indicated phenotype and the total number examined (denominator). **P<0.05.