FIGURE

Fig. 7

ID

ZDB-FIG-151117-14

Publication

Wu et al., 2015 - Control of Wnt5b secretion by wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression

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Fig. 7

Wntless controls chondrogenic cell proliferation during jaw cartilage development. (A–L) Confocal images showing lateral views of the heads of control-MO-injected (5mis-wls^MO) (A–F) or wls-MO-injected (wls^MO) (G–L) embryos stained by anti-Wls antibody (green in A,C,E,G,I,K) plus TUNEL (red in A-B,G-H), anti-pH3 (red in C,D,I,J) or anti-BrdU (red in E,F,K,L). The dotted lines of each triangle are defined as described in the Materials and Methods. The unstained regions indicate the the left eyes of the embryos. (M–X) Confocal images showing lateral view of the heads of WT (M–R) or wnt5b mutant (G–L) embryos stained by anti-Wls antibody (green in M,O,Q,S,U,W) plus TUNEL (red in M,N,S,T), anti-pH3 (red in O,P,U,V) or anti-BrdU (red in Q,R,W,X). (Y) The schematic on the left shows the three lines we defined to mark the region for counting the proliferating or apoptotic cells in the developing jaw (see Materials and Methods). (a–f) Statistical charts represent the quantitative results of apoptotic (TUNEL) or proliferating cells (pH3 or BrdU positive) at 48 hpf. **P<0.05. The n value is indicated. cMO, 5mis-wls^MO control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt5b^ti265/ti265 AB + MO1-wls
Knockdown Reagent:	MO1-wls
Observed In:	pharyngeal arch apoptotic process pharyngeal arch cartilage development pharyngeal arch cell population proliferation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.