ZFIN ID: ZDB-FIG-151106-30
Ningappa et al., 2015 - The Role of ARF6 in Biliary Atresia. PLoS One   10:e0138381 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Pec-fin to Day 5

Fig. 3

arf6 regulates intrahepatic biliary morphogenesis.

(A-C) Confocal images showing the development of the intrahepatic biliary network. Tg(Tp1:GFP) embryos/larvae were processed for immunostaining with anti-GFP (green). For 40 and 48 hpf, anti-Prox1 staining (red) was also used. Brackets delineate the length of BEC filopodia at 62, 72, and 96 hpf, and the length of interconnecting bile preductules at 5 dpf (A); graphs show their quantitation (B, C). (D) Confocal images showing the location of BEC nuclei in the entire liver. The Tg(Tp1:H2B-mCherry) line reveals BEC nuclei. Dashed lines outline clusters with four or more BECs. Graph shows the percentage of BECs present as single cells, doublets, triplets, or in clusters of four or more cells. (E) Confocal images of the liver immunostained for GFP (green) and Abcb11 (red). The Tg(Tp1:GFP) line and anti-Abcb11 reveal the intrahepatic biliary structure and bile canaliculi, respectively. All dotted lines outline the liver. Asterisks, statistical significance (* p<0.0001); error bars, ± SEM; scale bars, 25 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP um14Tg standard conditions Day 5 intrahepatic bile duct IFL
um14Tg + MO1-arf6a,arf6b standard conditions Day 5 intrahepatic bile duct IFL
mCherry s939Tg standard conditions Day 5 cholangiocyte nucleus IFL
s939Tg + MO1-arf6a,arf6b standard conditions Day 5 cholangiocyte nucleus IFL
prox1a Ab3-prox1 um14Tg standard conditions Prim-25 to Long-pec hepatoblast IHC
Ab3-prox1 um14Tg + MO1-arf6a,arf6b standard conditions Prim-25 to Long-pec hepatoblast IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-abcb11 IHC um14Tg standard conditions Day 5 bile canaliculus
IHC um14Tg + MO1-arf6a,arf6b standard conditions Day 5 bile canaliculus
Ab10-GFP IHC um14Tg standard conditions Prim-25 - Day 5 intrahepatic bile duct epithelial cell
IHC um14Tg + MO1-arf6a,arf6b standard conditions Prim-25 - Day 5 intrahepatic bile duct epithelial cell
Phenotype Details
Fish Conditions Stage Phenotype
s939Tg + MO1-arf6a,arf6b standard conditions Day 5 cholangiocyte aggregated, abnormal
um14Tg + MO1-arf6a,arf6b standard conditions Pec-fin - Day 5 cholangiocyte decreased amount, abnormal
Protruding-mouth - Day 4 cholangiocyte filopodium decreased length, abnormal
Day 5 bile canaliculus decreased length, abnormal
Day 5 bile ductule decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One