FIGURE

Fig. 6

ID

ZDB-FIG-151022-9

Publication

Mateus et al., 2015 - Control of tissue growth by Yap relies on cell density and F-actin in zebrafish fin regeneration

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Fig. 6

Differential PD expression of F-actin associates with Yap intracellular location. (A-F) Representative immunofluorescence with anti-Yap antibody and phalloidin (F-actin) in 72hpa longitudinal sections. (A) F-actin; (B) corresponding Yap expression. (C-F) High magnification images of the boxed areas in A,B showing F-actin (C,E) and Yap (D,F) localization in proximal (E,F) and distal (C,D) regions. Single color and merged xz projections of distal (C,D) and proximal (E,F) blastemas highlight intracellular localization. (G,H) Corresponding proximal and distal intensity profiles (in arbitrary units, a.u.) of xz projections represented showing average intensity of F-actin (G) and Yap (H). (I) Quantification changes in intracellular localization of Yap and F-actin across the PD axis by expressing a ratio between average intensities of proximal:distal Yap or F-actin of xz projections of respective mesenchymal cells. Mean±s.d. are shown. Dashed lines indicate amputation plane. n=7 sections, 5 fish. Scale bars: 50µm.

Expression Data

Gene:	yap1
Antibody:	Ab2-yap1
Fish:	AB
Condition:	amputation: caudal fin
Anatomical Terms:	blastema cytoplasm caudal fin nucleus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	amputation: caudal fin
Observed In:	blastema cytoplasm
Stage:	Adult

Phenotype Detail

Acknowledgments

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