header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150915-54
Gao et al., 2015 - TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. PLoS Genetics   11:e1005346 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1 The definitive hematopoiesis is defective in zebrafish mutantcas003 embryos.

(A, A′) The bright field images of zebrafish wild-type sibling (A) and mutantcas003 embryos (A′) showing no obvious difference at 5dpf. (B-G′) Whole-mount in situ hybridization (WISH) results of c-myb, ae1-globin, mpx, lyz and rag1 showing defective definitive hematopoiesis in mutantcas003 embryos (B′-G′) but not in sibling embryos (B-G) at 5dpf. The penetrance of the indicated phenotype is shown in the bottom left of each panel. (B-F, B′-F′) Lateral views; (G, G′) dorsal views. Black arrows indicate the position of caudal hematopoietic tissue (CHT); red arrows and circles show the position of thymus and kidney, respectively.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hbae1.1 WT control Day 5 hematopoietic system nucleate erythrocyte ISH
Day 5 nucleate erythrocyte ISH
topbp1cas003/cas003 control Day 5 hematopoietic system nucleate erythrocyte ISH
Day 5 nucleate erythrocyte ISH
lyz WT control Day 5 kidney macrophage ISH
Day 5 macrophage ISH
topbp1cas003/cas003 control Day 5 kidney macrophage ISH
Day 5 macrophage ISH
mpx WT control Day 5 granulocyte ISH
Day 5 hematopoietic system granulocyte ISH
Day 5 kidney granulocyte ISH
topbp1cas003/cas003 control Day 5 granulocyte ISH
Day 5 hematopoietic system granulocyte ISH
Day 5 kidney granulocyte ISH
myb WT control Day 5 hematopoietic system posterior side ISH
Day 5 kidney ISH
Day 5 thymus ISH
topbp1cas003/cas003 control Day 5 hematopoietic system posterior side ISH
Day 5 kidney ISH
Day 5 thymus ISH
rag1 WT control Day 5 thymus ISH
Day 5 thymus lymphocyte ISH
topbp1cas003/cas003 control Day 5 thymus ISH
Day 5 thymus lymphocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
topbp1cas003/cas003 control Day 5 definitive hemopoiesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.