FIGURE

Fig. 5

ID

ZDB-FIG-150915-58

Publication

Gao et al., 2015 - TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

The apoptosis of HSPCs is p53 dependent in topbp1^cas003 mutants.

(A) Quantitative PCR results of c-myb, p53, p21, cyclin G1 and mdm2 in the CHT region of wild-type sibling and topbp1^cas003 mutant embryos at 3dpf. All these p53 dependent apoptosis-related genes are upregulated. Error bars represent SEM. *, p<0.05; ***, p<0.001. (B) Quantitative analysis of HSPCs phenotype, monitored by c-myb WISH analysis, showing inactivation of p53 can partially rescue c-myb expression in topbp1^cas003 mutants. The 3 kinds of standards of c-myb WISH results in the CHT at 4dpf are shown in the right bottom. The numbers of embryos are shown above the columns. (C-F′) c-myb WISH analysis of siblings, topbp1^cas003, topbp1^cas003; p53^+/- and topbp1^cas003; p53^-/- embryos at 4dpf, which are quantified in B. E and E′ represent partially rescued embryos; F and F′ represent well rescued embryos. (C-D′, C-D′) 23/25 siblings and 8/9 topbp1^cas003 mutants show indicated phenotype. (C′-F′) Enlarged views of CHT region indicated by arrows in the left column.

Expression Data

Gene:	myb
Fish:	WT topbp1^{cas003/cas003} topbp1^{cas003/cas003}; tp53^zdf1/+ topbp1^{cas003/cas003}; tp53^zdf1/zdf1
Anatomical Terms:	hematopoietic multipotent progenitor cell hematopoietic system posterior side
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.