FIGURE

Fig. 4

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ZDB-FIG-150819-4

Publication

Gao et al., 2015 - Mecp2 regulates neural cell differentiation by suppressing the Id1-Her2/Hes5 axis in zebrafish

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Fig. 4

mecp2 negatively regulates her2 expression to control neural cell differentiation. (A–D′′) neurod, huc and islet1 decreased in mecp2 morphants (A′–D′), and this was partially rescued by co-injection of her2-MO (A′′–D′′), compared with controls (A–D). The expression of neurod increased in her2 morphants (A′′′,B′′′), compared with the wild-type (WT) (A,B). Dorsal views, with anterior to the left (A–D′). The numbers in the bottom right corner represent the number of embryos showing the indicated phenotype/total embryos examined. (E) Quantitative real-time PCR analysis of related gene expression in wild-type embryos and her2 morphants, normalized to gapdh. The data represent the mean±s.d. of three independent experiments. *P<0.05.

Expression Data

Genes:	EGFP elavl3 gfap GFP map2 nes neurod1
Antibody:	Ab1-elavl
Fish:	WT WT + MO2-mecp2 WT + MO3-her2 nl1Tg rw0Tg nl1Tg + MO2-mecp2 nl1Tg + MO3-her2 rw0Tg + MO2-mecp2 WT + MO2-mecp2 + MO3-her2 nl1Tg + MO2-mecp2 + MO3-her2 rw0Tg + MO2-mecp2 + MO3-her2
Knockdown Reagents:	MO2-mecp2 MO3-her2
Anatomical Terms:	brain brain neuron facial ganglion motor neuron trigeminal ganglion vagal ganglion
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-mecp2 WT + MO3-her2 nl1Tg + MO2-mecp2 nl1Tg + MO3-her2 rw0Tg + MO2-mecp2 WT + MO2-mecp2 + MO3-her2 nl1Tg + MO2-mecp2 + MO3-her2 rw0Tg + MO2-mecp2 + MO3-her2
Knockdown Reagents:	MO2-mecp2 MO3-her2
Observed In:	brain neuron differentiation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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