FIGURE

Fig. S4

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ZDB-FIG-150819-11

Publication

Gao et al., 2015 - Mecp2 regulates neural cell differentiation by suppressing the Id1-Her2/Hes5 axis in zebrafish

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Fig. S4

Overexpression of id1-mRNA mimics the phenotype in mecp2 morphants while id1-MO mimics that in embryos over-expressing mecp2 mRNA. RNA in situ hybridization with nestin, gfap, map2, and neurod probes in controls (A-D), embryos injected with id1-mRNA (A′-D′), embryos injected with mecp2-mRNA (A′′-D′′), and id1 morphants (A′′′-D′′′) at 48 hpf. The expression of these genes in the developing brain of embryos injected with id1-mRNA was similar to those in mecp2 morphants. The expression of nestin and gfap were down-regulated, while map2 and neurod were up-regulated in either embryos injected with mecp2-mRNA or id1 morphants. Dorsal views with anterior to the left. The numbers in the bottom right corner represent phenotypic embryos/total embryos. (E-G) Quantitative real-time PCR analysis of nestin, gfap, map2, and neurod gene expression in wild-type embryos along with embryos injected with mecp2-mRNA (E), id1 morphants (F), and embryos injected with id1-mRNA (G), normalized to gapdh. Measurements are the means from 3 independent experiments ± SD. *P <0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.