FIGURE

Fig. 2

ID
ZDB-FIG-150819-2
Publication
Gao et al., 2015 - Mecp2 regulates neural cell differentiation by suppressing the Id1-Her2/Hes5 axis in zebrafish
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Fig. 2

Knockdown of mecp2 increases neural precursors and astrogenesis while suppressing neurogenesis. (A,A2) RNA in situ hybridization performed with nestin probe in controls (wild-type, WT) (A) and mecp2 morphants (A′) at 48hpf. Note the upregulated nestin expression in the developing brain of mecp2 morphants. (B–E′) mecp2-MOs were injected into Tg(ef1α:mAG-zGem) transgenic embryos, in which GFP-positive cells are in the S, G2 and M phase of the cell cycle. Immunostaining was performed using an anti-pH3 antibody (red). The numbers of green fluorescence-positive cells and pH3-positive mitotic cells were increased in mecp2 morphants at 48hpf (C′,D′,E′) compared with the wild-type (C,D,E). Numbers of proliferative cells labeled by Tg(ef1α:mAG-zGem) were counted using Imaris software (B). Dorsal views are shown with anterior to the left (A,A&prime& C–E′). (F–H′) RNA in situ hybridization revealed that gfap expression was increased in the developing brain of mecp2 morphants (F′), compared with the wild-type (F) at 48hpf. However, map2 and neurod expression was reduced in mecp2 morphants (G′,H′) compared with controls (G,H) at 48hpf. (I,I′) mecp2-MOs were injected into Tg(neurod:EGFP) transgenic embryos. Note that neurod was downregulated in mecp2 morphants (I′), compared with controls (I) at 48hpf. (J,J′) Whole-mount immunostaining with antibody against Huc. Huc was downregulated in mecp2 morphants (J′), compared with controls (J) at 48hpf. (K,K′) mecp2-MOs were injected into Tg(islet1:EGFP) transgenic embryos, in which GFP-positive cells are motor neurons. The number of GFP-positive cells was reduced in mecp2 morphants (K′), compared with the wild-type (K) at 48hpf. Dorsal views are shown with anterior to the left (F–K′). (L) Quantitative real-time PCR analysis of related genes in wild-type embryos and mecp2 morphants, normalized to gapdh. Measurements are the mean±s.d. from three independent experiments. *P<0.05. In A,A′, and C–K′, numbers in the bottom right corner represent the number of embryos showing the indicated phenotype/total embryos examined.

Expression Data
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.