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ErbB signaling is implicated in generation of neurons and mitoses in the sub-ventricular zone. A. Treatment with an ErbB inhibitor AG1478 prevents generation of GFP-expressing neurons in the OT of Tg(pou4f1-hsp70l:GFP) embryos, but not with the control AG43 and DMSO, at 52 hpf. Dotted circle, OT. Scale bar, 100 µm. B. Quantification of pou4f1-hsp70l:GFP intensity in the OT for the experiment shown in A (mean ± s.e.m.; **P < 0.01, ***P < 0.001; n = 8, 10, 16 for AG1478, AG43, DMSO, respectively). C. (left) A timeline of AG1478 treatment and irradiation of UV light on Tg(elavl3:Kaede). (right) Representative embryos showing impaired generation of neurons in the OT (dotted circle) at 52 hpf following AG1478 treatment. Scale bar, 50 µm. D. WISH of embryos at 44 hpf. Expression of neurog1 and neurod is increased and diminished, respectively, while expression of her6 is obviously unaltered in the OT following AG1478 treatment. Scale bar, 100 µm. E. A coronal section of WISH-stained embryos for neurog1 mRNA at 36 hpf. F. Impaired neurogenesis and a decrease in pH3-positive mitotic cells in the SVZ of AG1478-treated Tg(pou4f1-hsp70l:GFP;-8.4neurog1:nRFP) embryos (AG1478) compared to the control embryos (DMSO) at 40 hpf. Scale bar, 10 µm. G. Quantification of the number of pH3-positive cells in the OT for the experiment shown in F, in the VZ (blue) and the SVZ (red) of AG1478-treated embryos and the control (white) embryos (mean ± s.e.m.; *P < 0.05; n = 11, 7 for AG1478, DMSO, respectively).
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