Fig. S8

Immunostained signals for Tyr¹¹⁶²-phosphorylated ErbB4 are diminished in the optic tectum by injection of MO^erbb4atg.

A. Alignment of amino acid sequences of human ERBB4 and zebrafish ErbB4 around Tyr¹¹⁸⁸ of human ERBB4. Identical amino acids are indicated by shaded boxes. A tyrosine residue recognized by the anti-phospho-HER4 pTyr1188 (pErbB4) antibody is shown in red. B. Immunohistochemical staining of cryosections of Tg(pou4f1-hsp70l:GFP) embryos at 48 hpf with the anti-pErbB4 antibody. Embryos were injected with MO^erbb4atg (right) or with standard control MO^ctrl (left). Yellow dotted circle, optic tectum (OT); e, eye; v, ventricle. Images at higher magnification in the OT are shown below. The strong signals for pErbB4 in the basal region (red arrows) would be probably derived from ErbB4 localized in dendrites of neurons, because the signals are disappeared in embryos injected with MO^erbb4atg. A, apical; B, basal. Scale bars, 50 µm (top), 10µm (bottom). C. Quantification of volume of pErbB4 puncta in the OT for the experiment shown in B (mean ± s.e.m.; **P < 0.01, unpaired t test; n = 7–8). The strong signals in the basal region (B, red arrows) are not included in this analysis.