ZFIN ID: ZDB-FIG-150618-5
Marjoram et al., 2015 - Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   112(9):2770-5 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 5

Fig. S2 TgBAC(tnfa:GFP) expression in WT and uhrf1pd1092 larvae. (A) Whole-mount image of WT and uhrf1pd1092 mutants showing the specificity of TgBAC(tnfa:GFP) up-regulation in the intestine (yellow dotted lines). (B–G) Confocal images of cross-sections stained with phalloidin (red). (B and C) Both WT and mutant larvae have GFP-positive cells in the brain and eye. The white arrow (C) points to an area of increased GFP expression in the mutant. (D and E) WT and mutant larvae express TgBAC(tnfa:GFP) in the gills (arrows). (F and G) Scattered GFP-positive cells are detected in the liver of mutants (G) but not in WT siblings. White dotted lines encircle the anterior gut; mutants display elevated TgBAC(tnfa:GFP) in the epithelium. (Scale bars: 50 µm.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP pd1028Tg standard conditions Day 5 brain IHC
Day 5 eye IHC
Day 5 Not Detected gut epithelium anterior region IHC
Day 5 gut epithelium posterior region IHC
uhrf1pd1092/pd1092; pd1028Tg standard conditions Day 5 brain IHC
Day 5 eye IHC
Day 5 gut epithelium anterior region IHC
Day 5 gut epithelium posterior region IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA