ZFIN ID: ZDB-FIG-150618-6
Marjoram et al., 2015 - Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   112(9):2770-5 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S8 A newly identified allele of dnmt1 is similar to uhrf1 and displays elevated TgBAC(tnfa:GFP) expression. (A) cc39.3pd1093 (Middle), a mutant recovered from our forward genetic screen, had a similar phenotype to uhrf1pd1092 and failed to complement (Top) with dnmt1s872 (Bottom). (B) Confocal images of crosssections through WT and dnmt1pd1093 mutants revealed excessive cell shedding (arrows), luminal debris, and a flattened epithelium in mutants. (C and D) Elevated TgBAC(tnfa:GFP) expression was observed in the intestinal epithelium (encircled by a red dotted line) of dnmt1pd1093 mutants (Upper and Lower Right) but not WT larvae at 120 hpf. Red arrows point to the intestinal epithelium. The yellow frame indicates the plane of section for C and D, Lower. Crosssections (Lower) through WT (C) and dnmt1pd1093 mutants (D) revealed up-regulation of TgBAC(tnfa:GFP) in the intestinal epithelium of mutants. (E) dnmt1pd1093 mutants have increased TgBAC(tnfa:GFP) epithelial fluorescence (measured in arbitrary units, a.u.), decreased cell height, and increased cell shedding, similar to uhrf1pd1092 mutants. ***P < 0.001, ****P < 0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP dnmt1pd1093/pd1093; pd1028Tg control Day 5 gut epithelium anterior region IHC
pd1028Tg control Day 5 Not Detected gut epithelium anterior region IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dnmt1pd1093/pd1093 standard conditions Day 5 gut epithelium flattened, abnormal
Day 5 gut epithelium morphology, abnormal
dnmt1pd1093/pd1093; pd1028Tg control Day 5 intestinal epithelial cell decreased height, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA