ZFIN ID: ZDB-FIG-150422-2
Marjoram et al., 2015 - Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   112(9):2770-5 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 2

Intestinal inflammation in uhrf1pd1092 mutants. Increased neutrophil recruitment, neutrophil infiltration into the intestinal epithelium, and elevated proinflammatory cytokine expression is observed in the intestine of uhrf1pd1092 mutants. (A) Whole-mount images of WT (Upper) and mutant (Lower) 120 hpf larvae stained with sudan black to mark neutrophils. Insets show a higher magnification view of the intestine (red dotted line), and arrows point to individual neutrophils. (B) Quantification of neutrophils in the intestinal area in WT (black) and mutant (gray) larvae. (n = 21 WT; n = 25). (C) Confocal images of cross-sections showing neutrophil (red) localization in 120 hpf WT and uhrf1pd1092 mutant larvae expressing LysC:dsRed. White arrows indicate neutrophils, which have infiltrated into the intestinal epithelium (yellow dotted line) in mutants but are restricted to the mesenchyme of WT. (Scale bars: 50 ┬Ám.) (D) Relative expression (mutant/WT) of proinflammatory genes in whole larvae. All genes are significantly elevated in mutants compared with WT. (E) Relative expression (mutant/WT) of tnfa and mmp9 in sorted intestinal epithelial cells. tnfa is greatly up-regulated in the intestine of mutants. (F) TgBAC(tnfa:GFP) construction schematic. (G and G′) Whole-mount (G) and confocal image of cross-section (G′) of a 120 hpf WT TgBAC(tnfa:GFP) transgenic larvae. The black star in G highlights autofluorescence in the WT gut, whereas the arrow points to the anterior gut epithelium that is devoid of TgBAC(tnfa:GFP) expression. (H and H′) Whole-mount (H) and confocal image of cross-section (H′) of 120 hpf uhrf1pd1092 larva with TgBAC(tnfa:GFP) in intestinal epithelium. The arrow highlights elevated epithelial expression of TgBAC(tnfa:GFP) in the intestine. The yellow bracket indicates the plane of section for G2 and H2. *P < 0.05, **P < 0.01, ***P < 0.001. Show Less

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed2 nz50Tg standard conditions Day 5 mesenchyme neutrophil IHC
Day 5 neutrophil IHC
uhrf1pd1092/pd1092; nz50Tg standard conditions Day 5 intestinal epithelium neutrophil IHC
Day 5 neutrophil IHC
GFP pd1028Tg standard conditions Day 5 Not Detected gut epithelium anterior region IHC
Day 5 gut epithelium posterior region IHC
Day 5 intestinal epithelial cell IHC
uhrf1pd1092/pd1092; pd1028Tg standard conditions Day 5 gut epithelium anterior region IHC
Day 5 gut epithelium posterior region IHC
Day 5 intestinal epithelial cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
uhrf1pd1092/pd1092 standard conditions Day 5 intestine has extra parts of type neutrophil, abnormal
Day 5 intestine neutrophil increased amount, abnormal
uhrf1pd1092/pd1092; nz50Tg standard conditions Day 5 intestine has extra parts of type neutrophil, abnormal
Day 5 intestine neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA