ZFIN ID: ZDB-FIG-150422-3
Marjoram et al., 2015 - Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   112(9):2770-5 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 3

Microbiota presence enhances tnfa elevation in the uhrf1pd1092 gut epithelium but is not required for the development of an IBD-like phenotype. (A–C) Confocal images of cross-sections of conventionally raised (CVR) 120 hpf WT (A); CVR 120 hpf uhrf1pd1092 (B), and germ-free (GF) 120 hpf uhrf1pd1092 (C) larvae. The arrows in B and C mark cells being shed from the epithelium. (D) TgBAC(tnfa:GFP) fluorescence (measured in arbitrary units, a.u.) under CVR and GF conditions. (E) Cell height measurement under CVR and GF conditions. Cell height is unchanged between CVR and GF mutants. (F) Quantification of cell shedding. Cell shedding was not decreased in GF mutants. Phalloidin, red. (Scale bars: 50 µm.) (D–F: n = 13 WT CVR; n = 9 mutant CVR; n = 11 mutant GF). Bars represent mean ± SEM. Significant differences between the means were detected after an ANOVA. *P < 0.05, **P < 0.01, ***P < 0.0001, n.s., not significant. Show Less

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP pd1028Tg control Day 5 intestinal epithelial cell IHC
uhrf1pd1092/pd1092; pd1028Tg control Day 5 intestinal epithelial cell IHC
uhrf1pd1092/pd1092; pd1028Tg germ free Day 5 intestinal epithelial cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
uhrf1pd1092/pd1092; pd1028Tg control Day 5 intestinal epithelial cell decreased height, abnormal
uhrf1pd1092/pd1092; pd1028Tg germ free Day 5 intestinal epithelial cell decreased height, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA