ZFIN ID: ZDB-FIG-150612-1
Hall et al., 2013 - Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production. Cell Metabolism   18(2):265-278 Full text @ Cell Metab.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1

Macrophage-Lineage Cells Express irg1 in Response to Infection

(A) Temporal QPCR analysis of irg1 expression within infected larvae (relative to PBS-injected controls) throughout first hour of infection, n = 3 biological replicates (mean ± SD).

(B and C) Expression of irg1 within PBS-injected control and infected larvae at 1 hpi, respectively. Arrow marks hindbrain ventricle injection site. Inset, magnified view of boxed region of similarly treated larva (10× objective).

(D) Quantification of irg1+ cells within individual infected larvae at 10, 20, 30, 40, 50, and 60 mpi (mean ± SD).

(E and F) Expression of irg1 within infected larvae at 6 and 24 hpi, respectively.

(G–I) Dual expression analysis of irg1 and mpeg1 (G), apoeb (H), and lyz (I) within the midbrain/hindbrain region of infected larvae at 24 hpi.

(J and K) Expression of irg1 within infected control MO-injected and Irf8-depleted larvae, respectively, at 1 hpi.

(L) Quantification of irg1+ cells within individual infected larvae as shown in (J) and (K) (mean ± SD). All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (B). p < 0.0001. See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acod1 WT control Long-pec Not Detected hindbrain ISH
Long-pec Not Detected midbrain ISH
Long-pec Not Detected trunk ISH
Long-pec Not Detected vasculature ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec hindbrain ISH
Long-pec midbrain ISH
Long-pec trunk ISH
Long-pec vasculature ISH
Long-pec whole organism RTPCR
Protruding-mouth hindbrain ISH
Protruding-mouth macrophage ISH
Protruding-mouth microglial cell ISH
Protruding-mouth midbrain ISH
WT + MO1-irf8 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec hindbrain ISH
Long-pec macrophage ISH
Long-pec midbrain ISH
Long-pec trunk ISH
Long-pec vasculature ISH
apoeb WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth microglial cell ISH
lyz WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth neutrophil ISH
mpeg1.1 WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth macrophage ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec hindbrain acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec midbrain acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec trunk acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec vasculature acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec whole organism acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth hindbrain acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth macrophage acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth microglial cell acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Protruding-mouth midbrain acod1 expression increased amount, abnormal
WT + MO1-irf8 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec hindbrain acod1 expression amount, ameliorated
Long-pec macrophage acod1 expression amount, ameliorated
Long-pec midbrain acod1 expression amount, ameliorated
Long-pec trunk acod1 expression amount, ameliorated
Long-pec vasculature acod1 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell Metabolism for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell Metabolism, 18(2), Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S., Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production, 265-278, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.