ZFIN ID: ZDB-FIG-150612-2
Hall et al., 2013 - Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production. Cell Metabolism   18(2):265-278 Full text @ Cell Metab.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

Infection-Responsive Expression of irg1 within Macrophage-Lineage Cells Is Dependent on the Primary Response Transcription Factor C/ebpβ

(A and B) Expression of irg1 within infected DMSO (control)- and CHX-treated larvae, respectively, at 1 hpi.

(C) Quantification of irg1+ cells within individual infected larvae as shown in (A) and (B) (mean ± SD).

(D) Expression of cebpβ within DMSO (control)- and CHX-treated larvae, following infection or PBS injection, at 1 hpi.

(E and F) Dual expression analysis of irg1 and cebpβ within the midbrain/hindbrain and trunk, respectively, of infected larvae at 24 hpi.

(G and H) Expression of cebpβ within infected control MO-injected and Irf8-depleted larvae, respectively, at 1 hpi.

(I and J) Expression of irg1 within infected control MO-injected and C/ebpβ-depleted larvae, respectively, at 1 hpi.

(K) Quantification of irg1+ cells within individual infected larvae as shown in (I) and (J) (mean ± SD).

(L) Schematic illustrating proposed regulation of infection-responsive irg1 expression within macrophage-lineage cells. Asterisks, arrows, and arrowheads mark blood-specific, liver-specific, and gut-specific cebpβ expression, respectively. All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (A). Abbreviations: DA, dorsal aorta; PCV, posterior cardinal vein; p < 0.0001. See also Figure S2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acod1 WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec macrophage ISH
Protruding-mouth blood macrophage ISH
Protruding-mouth hindbrain macrophage ISH
Protruding-mouth midbrain macrophage ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: cycloheximide Long-pec macrophage ISH
WT + MO1-cebpb bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec hindbrain macrophage ISH
Long-pec macrophage ISH
Long-pec midbrain macrophage ISH
Long-pec trunk ISH
cebpb WT control Long-pec intestine ISH
Long-pec liver ISH
WT chemical treatment: cycloheximide Long-pec anatomical structure ISH
Long-pec blood ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood ISH
Long-pec intestine ISH
Long-pec liver ISH
Protruding-mouth blood macrophage ISH
Protruding-mouth hindbrain macrophage ISH
Protruding-mouth midbrain macrophage ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: cycloheximide Long-pec anatomical structure ISH
Long-pec blood ISH
WT + MO1-irf8 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood ISH
Long-pec intestine ISH
Long-pec liver ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: cycloheximide Long-pec anatomical structure cebpb expression increased amount, abnormal
Long-pec blood cebpb expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood cebpb expression increased amount, abnormal
Long-pec macrophage acod1 expression increased amount, abnormal
Protruding-mouth blood macrophage acod1 expression increased amount, abnormal
Protruding-mouth blood macrophage cebpb expression increased amount, abnormal
Protruding-mouth hindbrain macrophage acod1 expression increased amount, abnormal
Protruding-mouth hindbrain macrophage cebpb expression increased amount, abnormal
Protruding-mouth midbrain macrophage acod1 expression increased amount, abnormal
Protruding-mouth midbrain macrophage cebpb expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: cycloheximide Long-pec anatomical structure cebpb expression increased amount, abnormal
Long-pec blood cebpb expression increased amount, abnormal
Long-pec macrophage acod1 expression amount, ameliorated
WT + MO1-cebpb bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec hindbrain macrophage acod1 expression amount, ameliorated
Long-pec macrophage acod1 expression amount, ameliorated
Long-pec midbrain macrophage acod1 expression amount, ameliorated
Long-pec trunk acod1 expression amount, ameliorated
WT + MO1-irf8 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood cebpb expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell Metabolism for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell Metabolism, 18(2), Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S., Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production, 265-278, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.