Fig. 2

Infection-Responsive Expression of irg1 within Macrophage-Lineage Cells Is Dependent on the Primary Response Transcription Factor C/ebpβ

(A and B) Expression of irg1 within infected DMSO (control)- and CHX-treated larvae, respectively, at 1 hpi.

(C) Quantification of irg1⁺ cells within individual infected larvae as shown in (A) and (B) (mean ± SD).

(D) Expression of cebpβ within DMSO (control)- and CHX-treated larvae, following infection or PBS injection, at 1 hpi.

(E and F) Dual expression analysis of irg1 and cebpβ within the midbrain/hindbrain and trunk, respectively, of infected larvae at 24 hpi.

(G and H) Expression of cebpβ within infected control MO-injected and Irf8-depleted larvae, respectively, at 1 hpi.

(I and J) Expression of irg1 within infected control MO-injected and C/ebpβ-depleted larvae, respectively, at 1 hpi.

(K) Quantification of irg1⁺ cells within individual infected larvae as shown in (I) and (J) (mean ± SD).

(L) Schematic illustrating proposed regulation of infection-responsive irg1 expression within macrophage-lineage cells. Asterisks, arrows, and arrowheads mark blood-specific, liver-specific, and gut-specific cebpβ expression, respectively. All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (A). Abbreviations: DA, dorsal aorta; PCV, posterior cardinal vein; p < 0.0001. See also Figure S2.