ZFIN ID: ZDB-FIG-150612-3
Hall et al., 2013 - Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production. Cell Metabolism   18(2):265-278 Full text @ Cell Metab.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 3

Glucocorticoid Receptor-Mediated Signaling Helps Regulate Infection-Responsive irg1 Expression within Macrophage-Lineage Cells through Induction of cebpβ Expression

(A and B) Expression of irg1 within infected DMSO (control)- and RU-486-treated (250 µM) larvae, respectively, at 1 hpi.

(C) Quantification of irg1+ cells within individual infected larvae treated with DMSO (control), 125 µM RU-486, or 250 µM RU-486 (mean ± SD).

(D and E) Expression of irg1 within infected control MO-injected and Nr3c1-depleted larvae, respectively, at 1 hpi.

(F) Quantification of irg1+ cells within individual larvae as shown in (D) and (E) (mean ± SD).

(G and H) Expression of cebpβ within infected control MO-injected and Nr3c1-depleted larvae, respectively, at 1 hpi.

(I) Immunofluorescence detection of Nr3c1 (Alexa 546) and Mpeg1/EGFP (Alexa 488) within DAPI-stained infected macrophage-lineage marking Tg(mpeg1:EGFP) larvae at 1 hpi. Dashed line marks Nr3c1+ nucleus.

(J) Expression of irg1 and cebpβ within dexamethasone-, hydrocortisone-, and prednisolone-injected larvae at 2 hpi.

(K) Quantification of irg1+ cells within individual larvae as shown in (J) (mean ± SD).

(L) Schematic illustrating proposed regulation of infection-responsive irg1 expression within macrophage-lineage cells. Asterisks, arrows, and arrowheads mark blood-specific, liver-specific, and gut-specific cebpβ expression, respectively. All views anterior to left. Numbers represent frequency of larvae with displayed phenotype. Scale bar, 100 µm in (A). Abbreviations: p < 0.001; p < 0.0001; n.s., not significant. See also Figure S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acod1 WT chemical treatment: dexamethasone Long-pec Not Detected macrophage ISH
WT chemical treatment: cortisol Long-pec Not Detected macrophage ISH
WT chemical treatment: prednisolone Long-pec Not Detected macrophage ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec macrophage ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: mifepristone Long-pec macrophage ISH
WT + MO4-nr3c1 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec macrophage ISH
cebpb WT chemical treatment: dexamethasone Long-pec blood macrophage ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec macrophage ISH
WT chemical treatment: cortisol Long-pec blood macrophage ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec macrophage ISH
WT chemical treatment: prednisolone Long-pec blood macrophage ISH
Long-pec intestine ISH
Long-pec liver ISH
Long-pec macrophage ISH
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood macrophage ISH
Long-pec intestine ISH
Long-pec liver ISH
WT + MO4-nr3c1 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood macrophage ISH
Long-pec intestine ISH
Long-pec liver ISH
EGFP gl22Tg bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec macrophage IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: dexamethasone Long-pec blood macrophage cebpb expression increased amount, abnormal
Long-pec macrophage cebpb expression increased amount, abnormal
WT chemical treatment: cortisol Long-pec blood macrophage cebpb expression increased amount, abnormal
Long-pec macrophage cebpb expression increased amount, abnormal
WT chemical treatment: prednisolone Long-pec blood macrophage cebpb expression increased amount, abnormal
Long-pec macrophage cebpb expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood macrophage cebpb expression increased amount, abnormal
Long-pec macrophage acod1 expression increased amount, abnormal
WT bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium, chemical treatment: mifepristone Long-pec macrophage acod1 expression amount, ameliorated
WT + MO4-nr3c1 bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium Long-pec blood macrophage cebpb expression amount, ameliorated
Long-pec macrophage acod1 expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cell Metabolism for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell Metabolism, 18(2), Hall, C.J., Boyle, R.H., Astin, J.W., Flores, M.V., Oehlers, S.H., Sanderson, L.E., Ellett, F., Lieschke, G.J., Crosier, K.E., and Crosier, P.S., Immunoresponsive Gene 1 Augments Bactericidal Activity of Macrophage-Lineage Cells by Regulating β-Oxidation-Dependent Mitochondrial ROS Production, 265-278, Copyright (2013) with permission from Elsevier. Full text @ Cell Metab.