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ZFIN ID: ZDB-FIG-150506-57
Chou et al., 2014 - The Hemodynamically-Regulated Vascular Microenvironment Promotes Migration of the Steroidogenic Tissue during Its Interaction with Chromaffin Cells in the Zebrafish Embryo. PLoS One   9:e107997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 7 Interaction between interrenal steroidogenic and chromaffin cells in tnnt2a, klf2a, and mmp2 morphants and RGD-treated embryos during interrenal gland assembly.

Double ISH assays showing colocalization of ff1b (red) and dbh (black) transcripts in uninjected control embryos and tnnt2a morphants at (A, A&prime& B, B′) 36 hpf (n = 3 and 6, respectively), (C, C&prime& D, D′) 56 hpf (n = 3 and 5, respectively), and (E, E&prime& F, F′) 3 dpf (n = 17 and 15, respectively), and in (G, G′) klf2a (n = 9) and (H, H′) mmp2 (n = 8) morphants and (I, I′) RGD-treated embryos (n = 18) at 3 dpf. Ventral flat mount views are shown for representative embryos in each group, oriented with anterior at the top. Yellow arrowheads indicate chromaffin cell clusters that failed to converge at the interrenal area in tnnt2a morphants. Broken white lines indicate position of the midline. (J) Schematic representation of various phenotypic defects associated with interrenal organ assembly. Panels show ventral views of wild-type, mutant, morphant, and drug-treated embryos at 3 dpf, oriented with anterior at the top. Phenotypes depicted for cloche (clo) and fn1 mutants are based on previous reports [9], [21]. Abbreviations: notochord (NC), the third somite (3S), the fourth somite (4S).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbh WT control Prim-25 chromaffin cell ISH
Long-pec chromaffin cell ISH
Protruding-mouth chromaffin cell ISH
WT chemical treatment: pharmaceutical Protruding-mouth chromaffin cell ISH
WT + MO1-klf2a standard conditions Protruding-mouth chromaffin cell ISH
WT + MO1-tnnt2a standard conditions Prim-25 chromaffin cell ISH
Long-pec chromaffin cell ISH
Protruding-mouth chromaffin cell ISH
WT + MO3-mmp2 standard conditions Protruding-mouth chromaffin cell ISH
nr5a1a WT control Prim-25 interrenal primordium ISH
Long-pec interrenal primordium ISH
Protruding-mouth interrenal primordium ISH
WT chemical treatment: pharmaceutical Protruding-mouth interrenal gland ISH
Protruding-mouth interrenal primordium ISH
WT + MO1-klf2a standard conditions Protruding-mouth interrenal gland ISH
Protruding-mouth interrenal primordium ISH
WT + MO1-tnnt2a standard conditions Prim-25 interrenal primordium ISH
Long-pec interrenal primordium ISH
Protruding-mouth interrenal gland ISH
Protruding-mouth interrenal primordium ISH
WT + MO3-mmp2 standard conditions Protruding-mouth interrenal gland ISH
Protruding-mouth interrenal primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Protruding-mouth adrenal gland development process quality, abnormal
Protruding-mouth interrenal gland animal organ morphogenesis process quality, abnormal
WT + MO1-klf2a standard conditions Protruding-mouth adrenal gland development process quality, abnormal
Protruding-mouth interrenal gland animal organ morphogenesis process quality, abnormal
WT + MO1-tnnt2a standard conditions Protruding-mouth adrenal gland development process quality, abnormal
Protruding-mouth chromaffin cell mislocalised, abnormal
Protruding-mouth interrenal gland animal organ morphogenesis process quality, abnormal
WT + MO3-mmp2 standard conditions Protruding-mouth adrenal gland development process quality, abnormal
Protruding-mouth interrenal gland animal organ morphogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One