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ZIRC
ZFIN ID: ZDB-FIG-150506-51
Chou et al., 2014 - The Hemodynamically-Regulated Vascular Microenvironment Promotes Migration of the Steroidogenic Tissue during Its Interaction with Chromaffin Cells in the Zebrafish Embryo. PLoS One   9:e107997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1 Morphology of pronephros and interrenal tissue in the absence of blood flow.

(A) The interrenal steroidogenic tissue positive for 3β-Hsd activity forms an extension that protrudes toward the midline by 54 hpf (cell protrusions marked by red arrows), while kidney glomeruli delineated by wt1b: GFP expression (G, yellow arrowheads) assemble at the midline. Morphogenetic movements of kidney glomeruli and steroidogenic tissues are defective in the tnnt2a morphant. All panels show dorsal views of representative embryos. (B) Quantification of effects of tnnt2aMO injection on interrenal migration. The distance between the midline and the migrating tip of steroidogenic tissue was designated as positive if the migrating tip had not reached the midline, and negative if the tip had migrated across the midline. (C) Relative density of steroidogenic tissue, as assessed by 3β-Hsd activity staining in the ventral surface, in tnnt2a morphants compared to wild-type controls. The number of embryos in each group is indicated in parentheses in (B) and (C). Histograms with different letters above them are significantly different (ANOVA and Duncan′s multiple test, P<0.05). (D) Effect of camptothecin treatment from 48 to 57 hpf on 3β-Hsd activity in steroidogenic cells. A, anterior; P, posterior; L, left; R, right. Broken yellow lines indicate position of the midline. Abbreviations: glomerulus (G). Scale bar, 50 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP li1Tg control Prim-15 pronephric glomerulus IHC
Prim-15 pronephric tubule IHC
Long-pec pronephric glomerulus IHC
Long-pec pronephric tubule IHC
Protruding-mouth pronephric glomerulus IHC
Protruding-mouth pronephric tubule IHC
li1Tg + MO1-tnnt2a standard conditions Prim-15 pronephric glomerulus IHC
Prim-15 pronephric tubule IHC
Long-pec pronephric glomerulus IHC
Long-pec pronephric tubule IHC
Protruding-mouth pronephric glomerulus IHC
Protruding-mouth pronephric tubule IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: camptothecin Long-pec interrenal gland decreased size, abnormal
li1Tg + MO1-tnnt2a standard conditions Long-pec adrenal gland development process quality, abnormal
Long-pec blood circulation decreased process quality, abnormal
Long-pec heart contraction decreased process quality, abnormal
Long-pec interrenal gland position, abnormal
Long-pec pronephric glomerulus separated from pronephric glomerulus, abnormal
Long-pec pronephric glomerulus morphogenesis process quality, abnormal
Protruding-mouth adrenal gland development process quality, abnormal
Protruding-mouth blood circulation decreased process quality, abnormal
Protruding-mouth heart contraction decreased process quality, abnormal
Protruding-mouth interrenal gland position, abnormal
Protruding-mouth pronephric glomerulus separated from pronephric glomerulus, abnormal
Protruding-mouth pronephric glomerulus morphogenesis process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One