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ZIRC
ZFIN ID: ZDB-FIG-150506-56
Chou et al., 2014 - The Hemodynamically-Regulated Vascular Microenvironment Promotes Migration of the Steroidogenic Tissue during Its Interaction with Chromaffin Cells in the Zebrafish Embryo. PLoS One   9:e107997 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagents:
Anatomical Term:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Pec-fin

Fig. 6 Steroidogenic cells are induced to undergo an EMT-like change by hemodynamic forces and pFak signaling.

(A–E) Ventral view of the midtrunk from 34 to 84 hpf; steroidogenic cells become loosely associated and develop protrusions at the cell surface. The accumulation of β-Catenin at cell-cell junctions in the steroidogenic tissue can be seen in cross sections of Tg(ff1bEx2: GFP) embryos at (F, F′) 2 dpf, but not at (G, G′) 2.5 dpf. The decrease in junctional β-Catenin was not observed in (H, H′) tnnt2a morphants, (I, I′) 2,3-BDM- or (J, J′) RGD-treated embryos, or (K, K′) klf2a or (L, L′) mmp2 morphants. Sections are shown of a representative embryo from each treatment group. (M) Fluorescence intensity of β-Catenin in ff1bGFP-expressing steroidogenic tissue (ROI marked by orange lines) is normalized to the size of the cluster, with the number of embryos indicated in parentheses. The difference between 2-dpf control group and any of the other groups was analyzed by Student′s t-test. *P<0.05, N.S., not significant. A, anterior; P, posterior; L, left; R, right; D, dorsal; V, ventral. Broken yellow lines indicate position of the midline. Abbreviations: notochord (NC). Scale bar, 25 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf346Tg control Long-pec interrenal primordium IHC
Pec-fin interrenal primordium IHC
zf346Tg chemical treatment: pharmaceutical Pec-fin interrenal primordium IHC
zf346Tg chemical treatment: pharmaceutical Pec-fin interrenal primordium IHC
zf346Tg + MO1-klf2a standard conditions Pec-fin interrenal primordium IHC
zf346Tg + MO1-tnnt2a standard conditions Pec-fin interrenal primordium IHC
zf346Tg + MO3-mmp2 standard conditions Pec-fin interrenal primordium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf346Tg chemical treatment: pharmaceutical Pec-fin interrenal gland epithelial to mesenchymal transition decreased occurrence, abnormal
zf346Tg chemical treatment: pharmaceutical Pec-fin interrenal gland epithelial to mesenchymal transition decreased occurrence, abnormal
zf346Tg + MO1-klf2a standard conditions Pec-fin interrenal gland epithelial to mesenchymal transition decreased occurrence, abnormal
zf346Tg + MO1-tnnt2a standard conditions Pec-fin interrenal gland epithelial to mesenchymal transition decreased occurrence, abnormal
zf346Tg + MO3-mmp2 standard conditions Pec-fin interrenal gland epithelial to mesenchymal transition decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One