FIGURE

Fig. 5

ID

ZDB-FIG-150428-5

Publication

Yang et al., 2015 - CNS Myelination Requires Cytoplasmic Dynein Function

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Fig. 5

Normal levels of CNS myelin gene expression require Dync1h1 function. A–F: Whereas 4 dpf wild-type larvae robustly express cldnk, plp1a, and mbp RNA, only apparently low levels of transcripts are evident in similarly processed dync1h1^vu76 mutant larvae. Images show dorsal views of whole larvae at the level of the midbrain and hindbrain, with anterior to the left. G,H: Quantitative RT-PCR confirmed the deficit of myelin gene expression in dync1h1^vu76 mutant larvae and dync1h1 MO-injected larvae. I,J: Levels of Mbp (red), detected by immunohistochemistry, appear higher in wild-type spinal cord than in mutant spinal cord. Anti-acetylated Tubulin (green) reveals axons. Images show transverse sections, with dorsal up. Statistical analysis was performed using an unpaired t test with Welsh correction. *P < 0.05; **P < 0.001. Error bars represent + SEM.

Expression Data

Genes:	cldnk mbpa plp1a
Antibodies:	Ab1-mbp Ab1-tuba
Fish:	WT dync1h1^vu76/vu76
Anatomical Terms:	central nervous system myelin sheath spinal cord axon spinal cord myelin sheath
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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