ZFIN ID: ZDB-FIG-150422-44
Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo. Development (Cambridge, England)   142:1050-61 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Protruding-mouth

Fig. 2

Zebrafish gata2b is expressed in hemogenic endothelium. (A) Expression of gata2b in whole embryos over time. cDNA isolated from 20-30 pooled embryos per timepoint. Error bars indicate s.e.m. of three biological samples. (B-G) Whole-mount in situ hybridization (WISH) of gata2b expression at the indicated stages. Yellow arrowheads indicate expression in the vascular cord region (C) and dorsal aorta (DA) (D,E). Blue arrowheads indicate branchiomotor neurons (D-F). Pink arrowheads indicate expression in the caudal hematopoietic tissue (CHT) (F,G). (H) qPCR of gata2b expression in GFP+ cells isolated from fli1a:GFP embryos by FACS. n.d., not detected. GFP+ cells sorted from 20-30 pooled embryos per timepoint. Error bars indicate s.e.m. of three technical replicates. (I) Transverse trunk sections showing WISH for gata2b at 18hpf. Top, rare cell detected outside the midline (asterisk). Bottom, representative section with gata2b+ cell localized to the midline. (J-M) Transverse sections showing WISH for gata2b (J), runx1 (K), kdrl (L) and gata2a (M) at 25hpf. Pink dashed ovals indicate the DA; orange dashed ovals indicate the posterior cardinal vein (PCV). Yellow arrowhead indicates a primitive erythrocyte. (N) Quantitation of gata2a and gata2b transcripts in kdrl+ cmyb and kdrl+ cmyb+ cells from 36hpf and 48hpf kdrl:mCherrymem;cmyb:GFP embryos. Cells isolated from 50-100 pooled embryos per sample. Two biological experiments were performed for each timepoint, with one representative shown. Error bars indicate s.e.m. of three technical replicates for each representative experiment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata2a WT control Prim-5 dorsal aorta ISH
Prim-5 erythroid lineage cell ISH
Prim-5 posterior cardinal vein ISH
s896Tg; zf169Tg control Prim-25 hematopoietic multipotent progenitor cell RTPCR
Prim-25 vascular endothelium RTPCR
Long-pec hematopoietic multipotent progenitor cell RTPCR
Long-pec vascular endothelium RTPCR
gata2b WT control 1-cell whole organism RTPCR
Bud Not Detected whole organism RTPCR
5-9 somites Not Detected whole organism RTPCR
14-19 somites vascular cord anatomical region ISH
14-19 somites whole organism RTPCR
20-25 somites branchiomotor neuron ISH
20-25 somites vascular cord anatomical region ISH
20-25 somites whole organism RTPCR
26+ somites whole organism RTPCR
Prim-5 branchiomotor neuron ISH
Prim-5 dorsal aorta ISH
Prim-5 ventral wall of dorsal aorta ISH
Prim-5 whole organism RTPCR
Long-pec branchiomotor neuron ISH
Long-pec hematopoietic cell ISH
Long-pec whole organism RTPCR
Protruding-mouth hematopoietic cell ISH
Protruding-mouth whole organism RTPCR
s896Tg; zf169Tg control Prim-25 hematopoietic multipotent progenitor cell RTPCR
Prim-25 vascular endothelium RTPCR
Long-pec hematopoietic multipotent progenitor cell RTPCR
kdrl WT control Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
runx1 WT control Prim-5 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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