FIGURE

Fig. 3

ID

ZDB-FIG-150422-45

Publication

Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Tg(gata2b:Gal4) marks hemogenic endothelial cells. Imaging is by confocal microscopy. (A) GFP⁺ endothelial cells in the trunk region of a gata2b:Gal4;UAS:GFP embryo at 24hpf. (B-C′) gata2b:Gal4;UAS:lifeactGFP at 30hpf and 3dpf. (C′) An enlarged view of C, with thymus and kidney highlighted. (D,D′) Transverse (D) and lateral (D′) views of the trunk vasculature of 30hpf gata2b:Gal4;UAS:GFP;kdrl:mCherry^mem. (E,E′) Transverse (E) and lateral (E′) views of a 48hpf gata2b:Gal4;UAS:GFP;kdrl:Cre;actB2:LoxP-STOP-LoxP-DsRedEx embryo showing a GFP⁺ cell undergoing EHT. (F,F′) A representative Kaede⁺ cell in the trunk of a gata2b:Gal4;UAS:Kaede embryo before (F) and after (F′) photoconversion. Blue dashed lines indicate the approximate location of DA and PCV. (G) Thymus of a 4dpf gata2b:Gal4;UAS:Kaede embryo that experienced photoconversion of a single aortic gata2b⁺ cell in the trunk at 46hpf. White dashed line highlights the thymus region. T, thymus; K, kidney; A, aorta; V, vein.

Expression Data

Genes:	DsRedx GFP Kaede mCherry
Fish:	hzm3Tg; s896Tg; sd32Tg hzm3Tg; s898Tg; sd32Tg; sd5Tg hzm3Tg; sd32Tg mu271Tg; sd32Tg rk8Tg; sd32Tg
Anatomical Terms:	dorsal aorta endothelial cell hematopoietic cell pronephros hematopoietic cell thymus hematopoietic cell vascular endothelium ventral wall of dorsal aorta ventral wall of dorsal aorta hematopoietic cell
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development