ZFIN ID: ZDB-FIG-150413-43
Jackson et al., 2015 - The role of Sox6 in zebrafish muscle fiber type specification. Skeletal muscle   5:2 Full text @ Skelet Muscle
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 4

tnnc1bis ectopically expressed in fast-twitch fibers of homozygoussox6mutants. Endogenous tnnc1b expression (A) and tnnc1b:eGFP reporter expression (green) (B) is restricted to slow-twitch fibers of wild-type embryos at 48 hours postfertilization (hpf) and excluded from fast-twitch fibers (B′:red) as shown in merged image (B′′). In sox6 mutants, endogenous tnnc1b(C) and the tnnc1b:eGFP reporter (D) are expressed in both slow and fast-twitch fibers (D′: red) as shown in merged image (D′′). Expression of ryr1a is restricted to slow-twitch fibers in wild type (E) but ectopically expressed in the fast-twitch fibers in sox6 mutant embryos at 30 hpf (I). By contrast, myh7b expression remains restricted to slow-twitch fibers in sox6 mutants (cf. F and J). Slow-twitch specific expression of the endogenous smyhc1(G) and the smyhc1:GFP reporter (H) in wild-type embryos. In sox6 mutants (K,L), expression is largely similarly restricted but ectopically expressed in a few fast fibers (arrows).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP i104Tg standard conditions Long-pec slow muscle cell IHC
i104Tg ; sox6i291/i291 standard conditions Long-pec fast muscle cell IHC
Long-pec slow muscle cell IHC
i293Tg standard conditions Long-pec slow muscle cell IHC
i293Tg ; sox6i291/i291 standard conditions Long-pec fast muscle cell IHC
Long-pec slow muscle cell IHC
myh7ba WT standard conditions Prim-15 slow muscle cell ISH
sox6i291/i291 standard conditions Prim-15 slow muscle cell ISH
ryr1a WT standard conditions Prim-15 slow muscle cell ISH
sox6i291/i291 standard conditions Prim-15 fast muscle cell ISH
Prim-15 slow muscle cell ISH
smyhc1 WT standard conditions Prim-15 slow muscle cell ISH
sox6i291/i291 standard conditions Prim-15 fast muscle cell ISH
Prim-15 slow muscle cell ISH
tnnc1b WT standard conditions Long-pec slow muscle cell ISH
sox6i291/i291 standard conditions Long-pec fast muscle cell ISH
Long-pec slow muscle cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F310 IHC i293Tg standard conditions Long-pec fast muscle cell
IHC i293Tg ; sox6i291/i291 standard conditions Long-pec fast muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
i104Tg ; sox6i291/i291 standard conditions Long-pec fast muscle cell EGFP expression mislocalised, abnormal
i293Tg ; sox6i291/i291 standard conditions Long-pec fast muscle cell EGFP expression mislocalised, abnormal
sox6i291/i291 standard conditions Prim-15 fast muscle cell ryr1a expression mislocalised, abnormal
Prim-15 fast muscle cell smyhc1 expression mislocalised, abnormal
Long-pec fast muscle cell tnnc1b expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Skeletal muscle for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Skelet Muscle