FIGURE

Fig. 4

ID

ZDB-FIG-150413-43

Publication

Jackson et al., 2015 - The role of Sox6 in zebrafish muscle fiber type specification

Other Figures

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Fig. 4

tnnc1bis ectopically expressed in fast-twitch fibers of homozygoussox6mutants. Endogenous tnnc1b expression (A) and tnnc1b:eGFP reporter expression (green) (B) is restricted to slow-twitch fibers of wild-type embryos at 48 hours postfertilization (hpf) and excluded from fast-twitch fibers (B′:red) as shown in merged image (B′′). In sox6 mutants, endogenous tnnc1b(C) and the tnnc1b:eGFP reporter (D) are expressed in both slow and fast-twitch fibers (D′: red) as shown in merged image (D′′). Expression of ryr1a is restricted to slow-twitch fibers in wild type (E) but ectopically expressed in the fast-twitch fibers in sox6 mutant embryos at 30 hpf (I). By contrast, myh7b expression remains restricted to slow-twitch fibers in sox6 mutants (cf. F and J). Slow-twitch specific expression of the endogenous smyhc1(G) and the smyhc1:GFP reporter (H) in wild-type embryos. In sox6 mutants (K,L), expression is largely similarly restricted but ectopically expressed in a few fast fibers (arrows).

Expression Data

Genes:	EGFP myh7ba ryr1a smyhc1 tnnc1b
Antibody:	Ab-F310
Fish:	WT sox6^i291/i291 i104Tg i293Tg sox6^i291/i291; i104Tg sox6^i291/i291; i293Tg
Anatomical Terms:	fast muscle cell slow muscle cell
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sox6^i291/i291 sox6^i291/i291; i104Tg sox6^i291/i291; i293Tg
Observed In:	fast muscle cell
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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