ZFIN ID: ZDB-FIG-150413-42
Jackson et al., 2015 - The role of Sox6 in zebrafish muscle fiber type specification. Skeletal muscle   5:2 Full text @ Skelet Muscle
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 3

Targeted mutagenesis of the zebrafishsox6locus. (A) Schematic representation of the zinc-finger nuclease designed to target genomic sequences in exon 8, upstream of the HMG box, of the zebrafish sox6 gene. (B-E) Immunohistochemical detection of Sox6 protein using a Sox6-specific antibody; Sox6 protein is detectable in the nuclei of fast muscle fibers (arrows) (B and B′) and the optic tectum (arrow) (C and C′) of wild-type embryos. In sox6 homozygous mutants, by contrast, no signal is detected in either the muscle (D and D′) or the optic tectum (E and E′), indicating the successful generation of a null mutant. (Blue signal = DAPI).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-sox6 IHC WT standard conditions Day 4 fast muscle cell nucleus
IHC Day 4 optic tectum
IHC sox6i291/i291 standard conditions Day 4 Not Detected fast muscle cell nucleus
IHC Day 4 Not Detected optic tectum
IHC sox6i292/i292 standard conditions Day 4 Not Detected fast muscle cell nucleus
IHC Day 4 Not Detected optic tectum
Phenotype Details
Fish Conditions Stage Phenotype
sox6i291/i291 standard conditions Day 4 fast muscle cell nucleus ab1-sox6 labeling absent, abnormal
Day 4 optic tectum ab1-sox6 labeling absent, abnormal
sox6i292/i292 standard conditions Day 4 fast muscle cell nucleus ab1-sox6 labeling absent, abnormal
Day 4 optic tectum ab1-sox6 labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Skeletal muscle for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Skelet Muscle