ZFIN ID: ZDB-FIG-150413-46
Jackson et al., 2015 - The role of Sox6 in zebrafish muscle fiber type specification. Skeletal muscle   5:2 Full text @ Skelet Muscle
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 7

Sox6 controls the Ca2+ response of fast-twitch fibers. (A,B) Cross section through a trunk somite of a 48 hours postfertilization (hpf) Tg(smyhc1:GCaMP3)i280 embryo, showing expression of GCaMP3 (green) is restricted to slow-twitch fibers, revealed by mAbF59 labeling (red). Nuclei are stained with DAPI (blue). (C) Stereotypical representation of the GCaMP3 signal in response to chemical stimulation indicating the three parameters measured in this analysis. (D) Response time in seconds (s) and (E) amplitude of Ca2+ response in fast and slow-twitch fibers of Tg(smyhc1:GCaMP3)i280 (Tg) and transient transgenic wild-type (+) and sox6 mutant larvae (52 to 55 hpf); Student’s two-tailed, unpaired t-test; Error bars represent standard deviation.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GCaMP i280Tg standard conditions Long-pec slow muscle cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC i280Tg standard conditions Long-pec slow muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
sox6i291/i291 ; i280Tg standard conditions Prim-15 fast muscle cell calcium-mediated signaling process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Skeletal muscle for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Skelet Muscle