FIGURE

Fig. 2

ID
ZDB-FIG-150402-19
Publication
Wan et al., 2014 - Retinal Injury, Growth Factors, and Cytokines Converge on β-Catenin and pStat3 Signaling to Stimulate Retina Regeneration
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

IGF-1 and FGF2 Synergize with Each Other to Stimulate MG Proliferation in the Uninjured Retina

(A) RT-PCR and qPCR analysis of injury-dependent induction of igf-1 mRNA. Error bars represent SD; p < 0.001; n = 6.

(B) In situ hybridization shows injury-dependent induction of igf-1 RNA in BrdU+ progenitors (arrows) at 4 dpi. Scale bar represents 50 µm. Asterisk marks the injury site.

(C and D) RT-PCR (C) and qPCR (D) analysis of igf1ra, igf1rb and igfbp3 gene expression in FACS purified MG and non-MG at different times post retinal injury. Error bars (D) represent SD; p < 0.05, p < 0.01; n = 4.

(E) BrdU immunofluorescence shows effect of morpholino (MO)-mediated knockdown of Igfbp3, Igfra, or Igfbp on progenitor formation in the injured retina. Scale bar represents 50 µm. Asterisk marks the injury site.

(F) Quantification of BrdU+ cells in (E). Error bars represent SD; p < 0.05; n = 3.

(G) BrdU immunofluorescence shows that conditional overexpression of dnFgfr1 in hsp70:dnfgfr1-egfp fish suppresses the generation of BrdU+ progenitors at 4 dpi. Asterisk marks the injury site. Scale bar represents 50 µm.

(H) Quantification of BrdU+ cells in (G). Error bars represent SD; p < 0.05 n = 3.

(I) BrdU immunofluorescence shows IGF-1 and FGF2 synergize to stimulate MG proliferation in the uninjured retina. Scale bar represents 50 µm. Bottom three panels show that BrdU+ cells are also GS+ MG.

(J) Quantification of BrdU+ cells in I. Error bars represent SD; n = 3.

See also Figure S2.

Expression Data
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.