ZFIN Image: Wan et al., 2014, Fig. 2

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Figure Caption

Fig. 2

IGF-1 and FGF2 Synergize with Each Other to Stimulate MG Proliferation in the Uninjured Retina

(A) RT-PCR and qPCR analysis of injury-dependent induction of igf-1 mRNA. Error bars represent SD; p < 0.001; n = 6.

(B) In situ hybridization shows injury-dependent induction of igf-1 RNA in BrdU⁺ progenitors (arrows) at 4 dpi. Scale bar represents 50 µm. Asterisk marks the injury site.

(C and D) RT-PCR (C) and qPCR (D) analysis of igf1ra, igf1rb and igfbp3 gene expression in FACS purified MG and non-MG at different times post retinal injury. Error bars (D) represent SD; p < 0.05, p < 0.01; n = 4.

(E) BrdU immunofluorescence shows effect of morpholino (MO)-mediated knockdown of Igfbp3, Igfra, or Igfbp on progenitor formation in the injured retina. Scale bar represents 50 µm. Asterisk marks the injury site.

(F) Quantification of BrdU⁺ cells in (E). Error bars represent SD; p < 0.05; n = 3.

(G) BrdU immunofluorescence shows that conditional overexpression of dnFgfr1 in hsp70:dnfgfr1-egfp fish suppresses the generation of BrdU⁺ progenitors at 4 dpi. Asterisk marks the injury site. Scale bar represents 50 µm.

(H) Quantification of BrdU⁺ cells in (G). Error bars represent SD; p < 0.05 n = 3.

(I) BrdU immunofluorescence shows IGF-1 and FGF2 synergize to stimulate MG proliferation in the uninjured retina. Scale bar represents 50 µm. Bottom three panels show that BrdU⁺ cells are also GS⁺ MG.

(J) Quantification of BrdU⁺ cells in I. Error bars represent SD; n = 3.