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ZIRC
ZFIN ID: ZDB-FIG-150331-15
Shao et al., 2009 - Down syndrome critical region protein 5 regulates membrane localization of Wnt receptors, Dishevelled stability and convergent extension in vertebrate embryos. Development (Cambridge, England)   136(12):2121-31 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Bud to Prim-5

Fig. 1

Overexpression or knockdown of dscr5 impairs CE movements. (A) Embryos were injected with dscr5 RNA or different MOs, as indicated. In situ hybridization was performed at 100% epiboly using the indicated probes, and live images were taken at 12.5 hpf. Upper panel, dorsoanterior view shows embryos simultaneously hybridized with dlx3, hgg1 and ntl probes; middle panel, dorsal view shows the expression domain of papc; bottom panel, lateral view shows the phenotypes of embryos at 12.5 hpf, with anterior to the top and dorsal side to the right. Arrowheads and arrows denote the anterior tip of the hypoblast and the tail region, respectively. (B) Summary of the dose-dependent effects of dscr5 knockdown using different MOs. Numbers at the top indicate total embryos scored at 27 hpf from three independent experiments. (C) Embryos were injected with a low dose of the indicated MOs and simultaneously hybridized with dlx3, hgg1 and ntl probes at 100% epiboly.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT control Bud prechordal plate ISH
WT standard conditions Bud prechordal plate ISH
WT + MO1-pigp standard conditions Bud prechordal plate ISH
WT + MO1-pigp + MO3-pigp standard conditions Bud prechordal plate ISH
WT + MO2-pigp standard conditions Bud prechordal plate ISH
WT + MO3-pigp standard conditions Bud prechordal plate ISH
dlx3b WT control Bud neural plate anatomical boundary ISH
WT standard conditions Bud neural plate anatomical boundary ISH
WT + MO1-pigp standard conditions Bud neural plate anatomical boundary ISH
WT + MO1-pigp + MO3-pigp standard conditions Bud neural plate anatomical boundary ISH
WT + MO2-pigp standard conditions Bud neural plate anatomical boundary ISH
WT + MO3-pigp standard conditions Bud neural plate anatomical boundary ISH
pcdh8 WT control Bud paraxial mesoderm ISH
WT standard conditions Bud paraxial mesoderm ISH
WT + MO1-pigp standard conditions Bud paraxial mesoderm ISH
WT + MO2-pigp standard conditions Bud paraxial mesoderm ISH
WT + MO3-pigp standard conditions Bud paraxial mesoderm ISH
tbxta WT control Bud notochord ISH
WT standard conditions Bud notochord ISH
WT + MO1-pigp standard conditions Bud notochord ISH
WT + MO1-pigp + MO3-pigp standard conditions Bud notochord ISH
WT + MO2-pigp standard conditions Bud notochord ISH
WT + MO3-pigp standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pigp standard conditions Bud convergent extension process quality, abnormal
Bud convergent extension involved in neural plate elongation process quality, abnormal
Bud neural plate increased width, abnormal
5-9 somites axis shortened, abnormal
5-9 somites convergent extension involved in axis elongation process quality, abnormal
Prim-5 convergent extension process quality, abnormal
WT + MO2-pigp standard conditions Bud convergent extension process quality, abnormal
Bud convergent extension involved in neural plate elongation process quality, abnormal
Bud neural plate increased width, abnormal
5-9 somites axis shortened, abnormal
5-9 somites convergent extension involved in axis elongation process quality, abnormal
Prim-5 convergent extension process quality, abnormal
WT + MO3-pigp standard conditions Bud convergent extension process quality, abnormal
Bud convergent extension involved in neural plate elongation process quality, abnormal
Bud neural plate increased width, abnormal
5-9 somites axis shortened, abnormal
5-9 somites convergent extension involved in axis elongation process quality, abnormal
Prim-5 convergent extension process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development