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ZFIN ID: ZDB-FIG-150325-19
Mommaerts et al., 2014 - Smoc2 modulates embryonic myelopoiesis during zebrafish development. Developmental dynamics : an official publication of the American Association of Anatomists   243(11):1375-90 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 8

smoc2 morphants display defects in embryonic myelopoiesis at the onset of blood circulation. A–D: Lateral views of the embryos, anterior to the left, dorsal to the top, at the onset of circulation. A: WISH analysis or the macrophage markers csf1ra (A) and irf8 (B), and the neutrophil markers c/ebp1 (C) and mpx (D). E–G: qPCR analysis for irf8 (E), c/ebp1 (F) and mpx (G). Values plotted as mean ± SEM; n = 4; *P < 0.05. H,I: Visualization of the mpx-positive cells using the mpx reporter embryos (Tg(mpx:GFP)i114) at 3 dpf (H) and the quantification of the number of neutrophils in the ATG morphants (n = 42) as compared to the control embryos (n = 12; *P < 0.05) (I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cebp1 WT control Prim-5 neutrophil ISH
WT + MO4-tp53 + MO5-smoc2 standard conditions Prim-5 neutrophil ISH
csf1ra WT control Prim-5 macrophage ISH
WT + MO4-tp53 + MO5-smoc2 standard conditions Prim-5 macrophage ISH
GFP i114Tg control Protruding-mouth neutrophil IFL
i114Tg + MO4-tp53 + MO5-smoc2 standard conditions Protruding-mouth neutrophil IFL
irf8 WT control Prim-5 macrophage ISH
WT + MO4-tp53 + MO5-smoc2 standard conditions Prim-5 macrophage ISH
mpx WT control Prim-5 neutrophil ISH
WT + MO4-tp53 + MO5-smoc2 standard conditions Prim-5 neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-tp53 + MO5-smoc2 standard conditions Prim-5 myeloid cell development process quality, abnormal
i114Tg + MO4-tp53 + MO5-smoc2 standard conditions Protruding-mouth neutrophil decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.