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ZIRC
ZFIN ID: ZDB-FIG-150202-12
Verleyen et al., 2014 - Orphan G-Protein Coupled Receptor 22 (Gpr22) Regulates Cilia Length and Structure in the Zebrafish Kupffer's Vesicle. PLoS One   9:e110484 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Day 4

Fig. 2

gpr22 deregulation results in defective LR patterning.

(A) Ventral or (B, C) dorsal views with anterior to the top of embryos at (A, B) 4 dpf or (C) 20 somite stage. (A) Transgenic embryos expressing GFP under control of the cmlc2 promoter. In contrast to water controls, gpr22 morphants show (from top to bottom) normal, absent, reversed or bicardial cardiac looping. Co-injection of 0.2 mM gpr22 MO and 100 pg/nl gpr22 RNA partially rescues the randomized cardiac looping (last panel). (B) WISH for foxa3. The liver of water control embryos develops at the left side of the embryo. In contrast, the position of the liver in embryos injected with 0.2 mM gpr22 MO is randomized (straight line). (C) WISH for southpaw (spaw). gpr22 morphants show randomized expression of the early left-specific LPM marker southpaw (straight line). (D) Quantifications of the heart or (E) liver/gut phenotype. MO = morpholino, V = ventricle, A = atrium, dpf = days post-fertilization, n = number of analyzed embryos, GFP = green fluorescence protein, cmlc2 = cardiac myosin light chain type 2, LR = left-right, WISH = whole mount in situ hybridization, LPM = lateral plate mesoderm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Day 4 heart IFL
twu34Tg + MO1-gpr22a + MO4-tp53 standard conditions Day 4 heart IFL
foxa3 WT control Day 4 gut ISH
Day 4 liver ISH
WT + MO1-gpr22a + MO4-tp53 standard conditions Day 4 gut ISH
Day 4 liver ISH
spaw WT control 20-25 somites lateral plate mesoderm left side ISH
WT + MO1-gpr22a + MO4-tp53 standard conditions 20-25 somites lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gpr22a + MO4-tp53 standard conditions 20-25 somites determination of left/right asymmetry in lateral mesoderm process quality, abnormal
WT + MO1-gpr22a + MO4-tp53 standard conditions 20-25 somites left/right axis specification process quality, abnormal
WT + MO1-gpr22a + MO4-tp53 standard conditions Day 4 determination of digestive tract left/right asymmetry process quality, abnormal
WT + MO1-gpr22a + MO4-tp53 standard conditions Day 4 determination of liver left/right asymmetry process quality, abnormal
WT + MO1-gpr22a + MO4-tp53 standard conditions Day 4 left/right axis specification process quality, abnormal
twu34Tg + MO1-gpr22a + MO4-tp53 standard conditions Day 4 heart looping process quality, abnormal
Day 4 left/right axis specification process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One