ZFIN ID: ZDB-FIG-150202-15
Verleyen et al., 2014 - Orphan G-Protein Coupled Receptor 22 (Gpr22) Regulates Cilia Length and Structure in the Zebrafish Kupffer's Vesicle. PLoS One   9:e110484 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 10-13 somites

Fig. 5

Gpr22 affects cilia length and structure in the KV.

(A–F) Immunostaining with anti-acetylated tubulin antibody on embryos at 10 somite stage injected at (A–D) one cell stage with (A) water, (B) 0.2 mM gpr22 MO, (C) 75 pg/nl gpr22 RNA, (D) 0.2 mM gpr22 MO and 100 pg/nl gpr22 RNA, or injected at (E, F) mid-blastula stage with (E) 1 mM control MO, (F) 1 mM gpr22 MO. Shown are the cilia of the KV. (G) Quantification of average cilia length ± s.d. (B, G) Whole embryo or (F, G) DFC-specific knock down of gpr22 significantly reduces cilia length. (C, G) In contrast, gpr22 overexpression results in significantly longer cilia. (D, G) Co-injection of 0.2 mM MO and 100 pg/nl RNA almost completely rescues cilia length. (H–J) TEM of cross sections of the KV cilia at 10 somite stage. (H) Structure of water control KV cilia showing 9 parallel outer microtubule doublets. (I) gpr22 knock down or (J) overexpression results in a disruption of the proper microtubuli arrangement or (I) even an absence of doublets. MO = morpholino, n = number of analyzed embryos, DFC = dorsal forerunner cells, DFCsuperscript = injected at mid-blastula stage, KV = Kupffer’s vesicle, TEM = transmission electron microscopy, * = P<0.01 (unpaired, two-tailed T-test).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gpr22a + MO4-tp53 standard conditions 10-13 somites Kupffer's vesicle cilium decreased length, abnormal
10-13 somites Kupffer's vesicle cilium disorganized, abnormal
10-13 somites Kupffer's vesicle cilium has fewer parts of type Kupffer's vesicle axonemal microtubule, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One