ZFIN ID: ZDB-FIG-150107-5
Maulding et al., 2014 - Mesodermal Fgf10b cooperates with other Fgfs during induction of otic and epibranchial placodes in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   243(10):1275-85 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 5

Effects of fgf10b knockdown on otic vesicle patterning. A-F: Lateral view (anterior to the left) of the otic vesicle (circled) at 24 hpf (A-D) and 36 hpf (E,F) labeled for the indicated genes. Arrows in (B) indicate the otic domain of pou3f3b.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT control Prim-5 otic vesicle dorsal region ISH
fgf3t26212/t26212 + MO2-fgf10b + MO3-fgf10b standard conditions Prim-5 otic vesicle dorsal region ISH
GFP fgf3t26212/t26212; s356tTg + MO2-fgf10b + MO3-fgf10b standard conditions Prim-25 otic sensory epithelium IFL
fgf3t26212/t26212; zc7Tg + MO2-fgf10b + MO3-fgf10b standard conditions Prim-25 statoacoustic (VIII) ganglion neuron IFL
s356tTg control Prim-25 otic sensory epithelium IFL
zc7Tg control Prim-25 statoacoustic (VIII) ganglion neuron IFL
otx1 WT control Prim-5 otic vesicle ventro-lateral region ISH
fgf3t26212/t26212 + MO2-fgf10b + MO3-fgf10b standard conditions Prim-5 otic vesicle ventro-lateral region ISH
pax5 WT control Prim-5 otic vesicle anterior region ISH
WT + MO2-fgf10b + MO3-fgf10b standard conditions Prim-5 otic vesicle anterior region ISH
fgf3t26212/t26212 standard conditions Prim-5 Not Detected otic vesicle ISH
fgf3t26212/t26212 + MO2-fgf10b + MO3-fgf10b standard conditions Prim-5 Not Detected otic vesicle ISH
pou3f3b WT control Prim-5 otic vesicle posterior region ISH
WT + MO2-fgf10b + MO3-fgf10b standard conditions Prim-5 otic vesicle posterior region ISH
fgf3t26212/t26212 standard conditions Prim-5 otic vesicle ISH
fgf3t26212/t26212 + MO2-fgf10b + MO3-fgf10b standard conditions Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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