ZFIN ID: ZDB-FIG-140821-10
Dong et al., 2014 - Pten regulates homeostasis and inflammation-induced migration of myelocytes in zebrafish. Journal of Hematology & Oncology   7:17 Full text @ J. Hematol. Oncol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 1

Definitive myelopoiesis is strongly expansive in pten-deficient embryos. (A-B) Whole-mount in situ hybridization analyses of definitive myelopoiesis with riboprobes for lyz in the indicated embryos at 90 hpf. The lower panels are high-magnification views of the indicated region in the upper panel. The red arrowheads indicate myeloid cells in the CHT and kidney/thymus region. (C-D) The pten-/-lyz:EGFP embryos show increased EGFP-positive myeloid cells. The red arrowheads indicate EGFP-positive cells in the CHT and kidney region. The embryos are shown in lateral views with the anterior to the left. e: eye. (E-F) Statistical result for a, b and c, d respectively. (G-M) Whole-mount in situ hybridization analyses for other myeloid markers (l-plastin, mpo, nephrosin) in the CHT. The red arrowheads indicate the myeloid cells marked by each probe. The data shown in (E-F) and (M) are the means ± SEM of at least 30 embryos; ***p < 0.001 versus the control. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ptenahu1864/hu1864; ptenbhu1435/hu1435; rj1Tg standard conditions Protruding-mouth myeloid cell IFL
rj1Tg standard conditions Protruding-mouth myeloid cell IFL
lcp1 WT standard conditions Protruding-mouth myeloid cell ISH
ptenahu1864/hu1864; ptenbhu1435/hu1435 standard conditions Protruding-mouth myeloid cell ISH
lyz WT standard conditions Protruding-mouth myeloid cell ISH
ptenahu1864/hu1864; ptenbhu1435/hu1435 standard conditions Protruding-mouth myeloid cell ISH
mpx WT standard conditions Protruding-mouth myeloid cell ISH
ptenahu1864/hu1864; ptenbhu1435/hu1435 standard conditions Protruding-mouth myeloid cell ISH
npsn WT standard conditions Protruding-mouth myeloid cell ISH
ptenahu1864/hu1864; ptenbhu1435/hu1435 standard conditions Protruding-mouth myeloid cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptenahu1864/hu1864; ptenbhu1435/hu1435 standard conditions Protruding-mouth myeloid cell increased amount, abnormal
Protruding-mouth myeloid cell differentiation process quality, abnormal
ptenahu1864/hu1864; ptenbhu1435/hu1435; rj1Tg standard conditions Protruding-mouth myeloid cell increased amount, abnormal
Protruding-mouth myeloid cell differentiation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Hematology & Oncology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Hematol. Oncol.