FIGURE

Fig. 1

ID

ZDB-FIG-140821-10

Publication

Dong et al., 2014 - Pten regulates homeostasis and inflammation-induced migration of myelocytes in zebrafish

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Fig. 1

Definitive myelopoiesis is strongly expansive in pten-deficient embryos. (A-B) Whole-mount in situ hybridization analyses of definitive myelopoiesis with riboprobes for lyz in the indicated embryos at 90 hpf. The lower panels are high-magnification views of the indicated region in the upper panel. The red arrowheads indicate myeloid cells in the CHT and kidney/thymus region. (C-D) The pten-/-lyz:EGFP embryos show increased EGFP-positive myeloid cells. The red arrowheads indicate EGFP-positive cells in the CHT and kidney region. The embryos are shown in lateral views with the anterior to the left. e: eye. (E-F) Statistical result for a, b and c, d respectively. (G-M) Whole-mount in situ hybridization analyses for other myeloid markers (l-plastin, mpo, nephrosin) in the CHT. The red arrowheads indicate the myeloid cells marked by each probe. The data shown in (E-F) and (M) are the means ± SEM of at least 30 embryos; ***p < 0.001 versus the control. Scale bar: 100 μm.

Expression Data

Genes:	EGFP lcp1 lyz mpx npsn
Fish:	WT rj1Tg ptena^{hu1864/hu1864}; ptenb^{hu1435/hu1435} ptena^{hu1864/hu1864}; ptenb^{hu1435/hu1435}; rj1Tg
Anatomical Term:	myeloid cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptena^{hu1864/hu1864}; ptenb^{hu1435/hu1435} ptena^{hu1864/hu1864}; ptenb^{hu1435/hu1435}; rj1Tg
Observed In:	myeloid cell myeloid cell differentiation
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Hematol. Oncol.