PUBLICATION

Pten regulates homeostasis and inflammation-induced migration of myelocytes in zebrafish

Authors
Dong, Z.W., Ren, C.G., Xia, Y., Su, D., Du, T.T., Fan, H.B., Yuan, H., Wang, L., Dong, M., Li, W.C., Jin, Y., Chen, Y., Deng, M., Liu, T.X., Gu, A.H., Zhou, Y.
ID
ZDB-PUB-140513-408
Date
2014
Source
Journal of Hematology & Oncology   7: 17 (Journal)
Registered Authors
Chen, Yi, Liu, Ting Xi, Zhou, Yong
Keywords
none
MeSH Terms
  • Animals
  • Apoptosis/physiology
  • Cell Growth Processes/physiology
  • Cell Movement/physiology*
  • Homeostasis
  • Inflammation/metabolism*
  • Myeloid Cells/cytology*
  • Myeloid Cells/metabolism*
  • PTEN Phosphohydrolase/metabolism*
  • Zebrafish
PubMed
24598081 Full text @ J. Hematol. Oncol.
Abstract
Loss of the tumor suppressor phosphatase and tensin homolog (PTEN) is frequently observed in hematopoietic malignancies. Although PTEN has been implicated in maintaining the quiescence of hematopoietic stem cells (HSCs), its role in hematopoiesis during ontogeny remains largely unexplored.
The expression of hematopoietic marker genes was analyzed via whole mount in situ hybridization assay in ptena and ptenb double mutant zebrafish. The embryonic myelopoiesis was characterized by living imaging and whole mount in situ immunofluorescence with confocal microscopy, as well as cell-specific chemical staining for neutrophils and macrophages. Analyses of the involved signaling pathway were carried out by inhibitor treatment and mRNA injection.
Pten-deficient zebrafish embryos exhibited a strikingly increased number of myeloid cells, which were further characterized as being immune deficient. In accordance with this finding, the inhibition of phosphoinositide 3-kinase (PI3K) or the mechanistic target of rapamycin (mTOR) corrected the expansive myelopoiesis in the pten-deficient embryos. Further mechanistic studies revealed that the expression of cebpa, a critical transcription factor in myeloid precursor cells, was downregulated in the pten-deficient myeloid cells, whereas the injection of cebpa mRNA markedly ameliorated the dysmyelopoiesis induced by the loss of pten.
Our data provide in vivo evidence that definitive myelopoiesis in zebrafish is critically regulated by pten via the elevation of cebpa expression.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping