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General Information
ZIRC
ZFIN ID: ZDB-FIG-140821-12
Dong et al., 2014 - Pten regulates homeostasis and inflammation-induced migration of myelocytes in zebrafish. Journal of Hematology & Oncology   7:17 Full text @ J. Hematol. Oncol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 3

The immune response of expansive myeloid cells is impaired in pten-/- embryos. (A and B) Tail transection analyses of myeloid cell movement in response to inflammation. The myeloid cells of the control and pten-/- embryos moved to the wound site at 6 hours post-transection (hpt) (90 hpf) (A and B, white boxes). The red arrowheads indicate the myeloid cells away from the wound (A and B, left region of the lower panels) and the myeloid cells that arrived at the wound (A and B, right region of the lower panels). A white asterisk indicates the heart edema of pten-/- embryos. (C-E) Quantification of myeloid cells in the region to the right of the white dashed line (A and B, lower panel) (C) and the total number of myeloid cells (D) in each embryo. (E) The percentage of myeloid cells that migrated to the wound. (F and G) Neutral Red staining of macrophages at 90 hpf. The black arrowheads indicate macrophages in the CHT. (H and I) Whole-mount in situ hybridization analyses of macrophages with riboprobe for c-fms. The black arrowheads indicate macrophages in the CHT. (J and K) Statistical results of F, G and H, I respectively. The data shown are the means ± SEM of at least 30 embryos; ***p < 0.001 versus the control. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
csf1ra WT physical alteration: anatomical structure Protruding-mouth macrophage ISH
ptenahu1864/hu1864; ptenbhu1435/hu1435 physical alteration: anatomical structure Protruding-mouth macrophage ISH
EGFP ptenahu1864/hu1864; ptenbhu1435/hu1435; rj1Tg physical alteration: anatomical structure Protruding-mouth myeloid cell IFL
rj1Tg physical alteration: anatomical structure Protruding-mouth myeloid cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ptenahu1864/hu1864; ptenbhu1435/hu1435 physical alteration: anatomical structure Protruding-mouth blood island has extra parts of type macrophage, abnormal
Protruding-mouth myeloid cell activation involved in immune response process quality, abnormal
ptenahu1864/hu1864; ptenbhu1435/hu1435; rj1Tg physical alteration: anatomical structure Protruding-mouth myeloid cell increased amount, abnormal
Protruding-mouth myeloid cell activation involved in immune response process quality, abnormal
Protruding-mouth neutrophil chemotaxis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Hematology & Oncology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Hematol. Oncol.