FIGURE

Fig. 2

ID

ZDB-FIG-140422-70

Publication

Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry

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Fig. 2

MZjek have a dysmorphic heart and reduced ventricular populations. (A,B) Lateral views of hearts stained with MF20 (red) and S46 (green) antibodies to visualize the ventricle and atrium of 48 hpf embryos. MF20 marks the entire heart and S46 is atrium specific. In these superimposed images MF20+S46- ventricle is red and MF20+S46+ atrium appears yellow. (A) Wildtype heart with two distinct chambers. (B) MZjek display a dysmorphic heart, with a misshapen atrium and a strung out ventricle (N=9). (C–F) amhc and vmhc expression in wildtype (C,E) and MZjek (D,F) at the 20-somite stage. MZjek mutants exhibit reduced expression of vmhc when compared to similarly staged wildtype embryos (N=25, 36 respectively).

Expression Data

Genes:	myh6 myh7
Antibodies:	Ab-MF20 Ab-S46
Fish:	WT ugdh^m310/m310
Anatomical Terms:	atrium heart heart rudiment
Stage Range:	20-25 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ugdh^m310/m310
Observed In:	atrium cardiac ventricle
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.