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ZFIN ID: ZDB-FIG-140319-45
Weidinger et al., 1999 - Identification of tissues and patterning events required for distinct steps in early migration of zebrafish primordial germ cells. Development (Cambridge, England)   126(23):5295-5307
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to 5-9 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 75%-epiboly to 5-9 somites

Fig. 5 The PGCs are found in random dorsoventral positions in dorsalized swirl mutants, but all are located at the same anteroposterior level. All embryos were stained with vasa in blue and a second staining in red was done with the probes indicated. (A,B) At the 1-somite stage, an alignment of PGCs anterior to trunk mesoderm (labeled by papc and myoD) is observed in lateral view of wild-type (A) and swirl mutant (B) embryos (anterior up, dorsal right), but PGCs are found all around the dorsoventral axis in swirl mutant embryos. (C,D) Lateral view of 5-somite-stage embryos stained with pax2.1 and myoD (here only the adaxial cells are labeled by this probe). PGCs are still dispersed around the circumference of the embryo in swirl mutants (D); they do not cluster together as they do in wild-type embryos at this stage (C) (see Fig. 1I for a dorsal view of wild-type). (E,F) PGCs have converged towards the dorsal in wildtype (E), but not swirl mutant (F) embryos at 80% epiboly. Embryos are shown in vegetal view with the dorsal marked by chordino expression in the wild-type (E) and circumferential chordino expression in the swirl mutant (F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd WT standard conditions 75%-epiboly whole organism dorsal region ISH
bmp2bta72a/ta72a(TU) standard conditions 75%-epiboly anatomical structure ISH
ddx4 WT standard conditions 75%-epiboly primordial germ cell ISH
1-4 somites primordial germ cell ISH
5-9 somites primordial germ cell ISH
bmp2bta72a/ta72a(TU) standard conditions 75%-epiboly primordial germ cell ISH
1-4 somites primordial germ cell ISH
5-9 somites primordial germ cell ISH
myod1 WT standard conditions 1-4 somites mesoderm ISH
5-9 somites adaxial cell ISH
bmp2bta72a/ta72a(TU) standard conditions 1-4 somites mesoderm ISH
5-9 somites adaxial cell ISH
pax2a WT standard conditions 5-9 somites immature eye ISH
5-9 somites midbrain hindbrain boundary neural plate ISH
5-9 somites otic placode ISH
5-9 somites pronephric mesoderm ISH
bmp2bta72a/ta72a(TU) standard conditions 5-9 somites immature eye ISH
5-9 somites midbrain hindbrain boundary neural plate ISH
5-9 somites otic placode ISH
5-9 somites pronephric mesoderm ISH
pcdh8 WT standard conditions 1-4 somites mesoderm ISH
bmp2bta72a/ta72a(TU) standard conditions 1-4 somites mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp2bta72a/ta72a(TU) standard conditions 75%-epiboly primordial germ cell mislocalised, abnormal
75%-epiboly whole organism wholly dorsalized, abnormal
1-4 somites primordial germ cell mislocalised, abnormal
1-4 somites whole organism wholly dorsalized, abnormal
5-9 somites primordial germ cell mislocalised, abnormal
5-9 somites whole organism wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.