FIGURE

Fig. 6

ID

ZDB-FIG-140319-46

Publication

Weidinger et al., 1999 - Identification of tissues and patterning events required for distinct steps in early migration of zebrafish primordial germ cells

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Fig. 6

PGC migration phenotype of knypek and floating head mutant embryos. All embryos were stained with vasa in dark blue and other probes in red or light blue as indicated. (A) Trailing PGCs align along the abnormally located pronephros (stained with pax2.1, adaxial cells and somites with myoD) in knypek mutant embryos at the 7- somite stage. (B,C) Normal arrangement of PGCs in wild-type (B) and floating head mutant (C) embryos at the 8-somite stage stained with myoD and pax2.1. (D,E) Dorsal view of embryos at the 90% epiboly stage showing that dorsally located PGCs do not migrate away from the dorsal midline in floating head mutant embryos. (D) In wild-type embryos, PGCs are not found at the region of the forming notochord marked by lack of papc staining, but they extend into the dorsal midline that expresses papc in floating head mutant embryos (E).

Expression Data

Genes:	ddx4 myod1 pax2a pcdh8
Fish:	WT gpc4^m818/m818 noto^tk241/tk241
Anatomical Terms:	adaxial cell immature eye mesoderm midbrain hindbrain boundary neural plate otic placode primordial germ cell pronephric mesoderm somite
Stage Range:	90%-epiboly to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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