ZFIN ID: ZDB-FIG-140228-49
Stainier et al., 1995 - cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. Development (Cambridge, England)   121:3141-3150
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to 26+ somites

Fig. 7 clo mutant embryos do not express GATA-1 or GATA-2 in their hematopoietic tissues. In situ hybridization with a GATA-1 or GATA-2 antisense RNA probe was performed on wild-type embryos (A,C,E) and on clo mutants (B,D,F). (A-D) GATA-1 staining at the 22-somite stage. In wild-type embryos, GATA-1 expression is originally found in two longitudinal chords of cells that extend from the mid-trunk region to just beyond the yolk tube and pronephric ducts. These cell populations will fuse at the midline to form the ICM as seen in whole mounts (A) and transverse sections of the tail region (C). clo mutants fail to express GATA-1 (B,D). (E,F) GATA-2 staining of 20-somite stage embryos. GATA-2 is normally expressed from an early stage in all hematopoietic progenitors including the presumptive stem cells of the posterior ICM (E, arrow). In clo mutants, the hematopoietic tissues do not express GATA-2 (F). GATA-2 is also expressed in certain neural tissues (Detrich et al., 1995) and this expression is normal in mutant embryos (data not included).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a WT standard conditions 26+ somites hematopoietic multipotent progenitor cell ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions 26+ somites Not Detected hematopoietic multipotent progenitor cell ISH
gata2a WT standard conditions 20-25 somites hematopoietic multipotent progenitor cell ISH
20-25 somites intermediate cell mass of mesoderm hematopoietic stem cell ISH
npas4lDf(Chr13:lycat)m39(INDO) standard conditions 20-25 somites Not Detected hematopoietic multipotent progenitor cell ISH
20-25 somites Not Detected intermediate cell mass of mesoderm hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.