ZFIN ID: ZDB-FIG-140228-44
Stainier et al., 1995 - cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. Development (Cambridge, England)   121:3141-3150
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 20-25 somites

Fig. 4 The endocardial progenitor cells are absent from the heart region in clo mutant embryos at the time of heart tube formation. Horizontal sections of a wild-type (A,B) and a clo mutant embryo (C,D) at the 24-somite stage. In wild type, tropomyosin immunoreactive myocardial cells (arrows) surround the endocardial progenitor cells as the two primitive myocardial tubes fuse to form the definitive heart tube (Stainier et al., 1993). In clo, tropomyosin immunoreactive myocardial cells (arrows) surround a space which is conspicuously empty and somewhat collapsed. (Serial sections were examined throughout this region in several mutant embryos and no endocardial progenitor cells were seen). In C and D, a more apical region of the heart cone (i.e. a more arterial region of the heart) is shown which explains why the mutant heart appears smaller than wild type. Scale bar, 50 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tpm IHC WT standard conditions 20-25 somites myocardium
IHC npas4lDf(Chr13:lycat)m39(INDO) standard conditions 20-25 somites myocardium
Phenotype Details
Fish Conditions Stage Phenotype
npas4lDf(Chr13:lycat)m39(INDO) standard conditions 20-25 somites endocardial precursor absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.